Pyronin Y | 92-32-0

Pyronin Y is a cationic dye commonly used in various scientific and clinical applications. Here are some key applications of Pyronin Y:

Nucleic Acid Staining: Pyronin Y is extensively used for staining RNA in cells and tissues. It selectively binds to RNA, allowing researchers to visualize and quantify RNA content under a microscope. This is particularly useful in histology and cytology for studying cell cycle dynamics and differentiation processes.

Flow Cytometry: In flow cytometry, Pyronin Y is employed to distinguish between quiescent and actively cycling cells. When used in combination with other DNA-binding dyes, it enables the identification of cells in different phases of the cell cycle. This information is critical for cancer research and cell cycle studies.

Bacterial Viability Testing: Pyronin Y can be utilized to assess bacterial viability by differentiating between live and dead cells. Live cells retain Pyronin Y, appearing red, whereas dead cells do not, making it possible to quickly evaluate the efficacy of antimicrobial treatments. This application is valuable in microbiological diagnostics and antibiotic testing.

Transcriptional Activity Measurement: Pyronin Y is also used to measure transcriptional activity in living cells. By staining RNA-rich regions, it reflects the levels of transcriptional activity, providing insights into gene expression patterns. This is particularly helpful in understanding cellular responses to different environmental conditions and treatments.