Trisulfo-Cy3-Alkyne | CAS 1895849-34-9

Trisulfo-Cy3-Alkyne is a Cy3-based fluorescent labeling reagent featuring a sulfonated, water-compatible dye scaffold and a terminal alkyne handle for copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. This combination supports robust conjugation workflows in aqueous bioconjugation settings, enabling fluorescent tagging of biomolecules, polymers, and biomaterial surfaces for microscopy and fluorescence readouts. The Cy3 emission profile makes it a widely used red/orange channel fluorophore for multicolor imaging and assay development.

1. Biomolecule Click Labeling

Trisulfo-Cy3-Alkyne is used by chemical biology and proteomics groups to fluorescently label azide-functional biomolecules through CuAAC click chemistry, including proteins, peptides, and other macromolecules prepared with azide groups. Researchers incorporate the dye to generate defined Cy3-tagged conjugates for localization studies, binding experiments, and fluorescence-based characterization of labeled biomolecules. The sulfonated dye design supports aqueous labeling workflows where dye solubility and consistent performance in buffered media are important for downstream imaging and quantification.

2. Fluorescence Microscopy Tracing

Trisulfo-Cy3-Alkyne is commonly applied in fluorescence microscopy workflows where a red/orange-emitting Cy3 fluorophore is needed to visualize click-labeled targets. Users generate fluorescently tagged conjugates (for example, azide-bearing targeting ligands, polymeric carriers, or biomaterial components) and then track their distribution in cells, extracellular matrices, or labeled biological assemblies. The terminal alkyne enables modular probe construction, allowing researchers to swap the azide partner to tailor labeling density and experimental context while keeping the imaging readout in a familiar Cy3 spectral channel.

3. Biomaterial And Surface Functionalization

Trisulfo-Cy3-Alkyne is frequently used in biomaterials research to fluorescently engineer azide-functional surfaces, hydrogels, and polymer coatings via click conjugation. Materials scientists employ the reagent to create traceable, Cy3-labeled interfaces for studying adsorption, coating uniformity, diffusion, and material-biomolecule interactions under fluorescence microscopy or plate-based fluorescence measurements. The water-compatible sulfonated dye scaffold helps maintain labeling performance during surface modification steps performed in aqueous conditions, supporting reproducible visualization of functionalized materials.

4. Click-Constructed FRET Pairs

Trisulfo-Cy3-Alkyne is also used in fluorescence assay development where Cy3 serves as a donor fluorophore in click-assembled FRET constructs. Researchers prepare azide-containing acceptors or FRET partners and then use the alkyne handle to generate defined donor-acceptor geometries through CuAAC, enabling ratiometric or emission-ratio readouts in biochemical and materials studies. This approach is particularly valuable when modular, orthogonal labeling is required to build FRET systems without changing the core fluorophore identity of the donor component.