Sulfo-Cyanine3 tetrazine

Sulfo-Cyanine3 tetrazine, a versatile fluorescent dye, finds widespread use in diverse biological and biochemical contexts. Explore four key applications of Sulfo-Cyanine3 tetrazine:

Bioorthogonal Labeling: Positioned at the intersection of bioorthogonal chemistry, Sulfo-Cyanine3 tetrazine excels in labeling biomolecules within living cells and organisms. This dye exhibits specific reactivity with trans-cyclooctene (TCO)-modified molecules, enabling precise and selective imaging of target proteins or nucleic acids. This sophisticated technique allows for real-time observation of dynamic biological processes devoid of cellular disruptions.

Fluorescence Microscopy: As a luminous and enduring fluorescent tag in fluorescence microscopy, Sulfo-Cyanine3 tetrazine illuminates cellular structures and molecular interactions with remarkable clarity. Researchers leverage this dye to visualize biomolecule localization, movement, and interactions in both fixed and live cell specimens, enriching our comprehension of intricate biological systems.

Protein Conjugation: Sulfo-Cyanine3 tetrazine plays a pivotal role in conjugating proteins, antibodies, and other biomolecules for diverse assays and detection systems. By coupling this dye to specific biomolecules, scientists can trace and quantify them across various experimental frameworks, such as Western blotting, flow cytometry, and ELISA.

Super-Resolution Imaging: Embracing cutting-edge super-resolution imaging techniques like STED (Stimulated Emission Depletion) microscopy, Sulfo-Cyanine3 tetrazine stands out for its exceptional photostability and luminosity. These attributes make it a fitting choice for achieving nanoscale resolution of cellular and molecular architectures. Empowered by this advanced imaging prowess, researchers can scrutinize biological specimens with unrivaled detail and precision.