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Sulfo-Cyanine5-PEG3-Biotin
| Catalog Number | F03-0028 |
| Category | sulfo-Cyanine3 |
| Molecular Formula | C48H65N6KO11S3 |
| Molecular Weight | 1037.36 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| F03-0028 | -- | $-- |
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Product Introduction
Sulfo-Cyanine5 is a far-red fluorophore widely used for biomolecule labeling, with excitation maximum at 646 nm and emission maximum at 662 nm. Far-red fluorescent tags with excitation above 600 nm and emission further than 650 nm are valuable for imaging techniques because of the lower background autofluorescence at these wavelengths. Besides, far-red fluorescent labels can be imaged simultaneously with near-red, orange, green, and blue tags, which is advantageous for multicolor imaging.,Biotin, or the water-soluble vitamin H, is well known not only for playing essential roles in various crucial metabolic cellular reactions but also for its extremely high affinity for avidin, a glycoprotein of egg white. Avidin, streptavidin (bacterial analogue of avidin), and neutravidin (deglycosylated avidin) bind non-cooperatively to biotin with high affinity. Thus, sulfo-Cyanine5 biotin conjugate can be used for detecting and quantifying biotin binding sites of avidin, streptavidin, and neutravidin in samples of different origin.,Sulfo-Cyanine5 biotin conjugate is a water-soluble reagent and its fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 linker between biotin moiety and fluorescent tag provides binding to avidin, streptavidin, or neutravidin without steric troubles.,The major applications of sulfo-Cyanine5 biotin conjugate include imaging (e.g. primary and secondary antibody labeling for Western Blotting, immunoassay, cyto- and histochemistry, flow cytometry), affinity and dissociation constant measuring, streptavidin-based sensors, etc.
Chemical Information |
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|---|---|
| Purity | NMR 1H, HPLC-MS (95%) |
| IUPAC Name | potassium;1-[6-[2-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethylamino]-6-oxohexyl]-3,3-dimethyl-2-[5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate |
| Canonical SMILES | CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=CC=C3C(C4=C(N3CCCCCC(=O)NCCOCCOCCNC(=O)CCCCC5C6C(CS5)NC(=O)N6)C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C.[K+] |
| InChI | InChI=1S/C48H66N6O11S3.K/c1-47(2)35-30-33(67(58,59)60)19-21-38(35)53(5)41(47)15-8-6-9-16-42-48(3,4)36-31-34(68(61,62)63)20-22-39(36)54(42)25-13-7-10-17-43(55)49-23-26-64-28-29-65-27-24-50-44(56)18-12-11-14-40-45-37(32-66-40)51-46(57)52-45;/h6,8-9,15-16,19-22,30-31,37,40,45H,7,10-14,17-18,23-29,32H2,1-5H3,(H5-,49,50,51,52,55,56,57,58,59,60,61,62,63);/q;+1/p-1/t37-,40-,45-;/m0./s1 |
| InChI Key | AFUPYAGXPYBXDE-DHAJKTQTSA-M |
| Solubility | Good in water, DMF, DMSO |
| Appearance | Dark Blue solid |
- Product Specification
- Application
| CF260 | 0.04 |
| CF280 | 0.04 |
| Fluorescence Quantum Yield | 0.28 |
| Excitation | 646 |
| Emission | 662 |
| Storage | Storage: 24 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Sulfo-Cyanine5-PEG3-Biotin, a distinguished fluorescent probe in molecular biology and biochemistry, distinguishes itself through exceptional photostability and bioconjugation properties. Explore four key applications of Sulfo-Cyanine5-PEG3-Biotin:
Fluorescence Imaging: Radiating a dazzling brilliance in fluorescence imaging, Sulfo-Cyanine5-PEG3-Biotin acts as a beacon for tagging biomolecules such as proteins, nucleic acids, and lipids. Its enduring luminosity enables precise visualization of cellular components with unparalleled clarity, significantly enhancing live-cell imaging and the meticulous examination of fixed cells.
Protein-Protein Interaction Studies: Unraveling the intricacies of protein interactions, Sulfo-Cyanine5-PEG3-Biotin emerges as a powerful tool for labeling one of the interacting partners. Through biotinylated binding assays combined with avidin or streptavidin-conjugated fluorophores, detection and quantification of these intricate interactions are made feasible. This technique stands at the forefront of deciphering complex biological processes and propelling innovations in drug discovery.
Flow Cytometry: Embark on a journey through flow cytometry with Sulfo-Cyanine5-PEG3-Biotin, a pivotal element in cell staining for analyzing various markers on cell surfaces or within cells. Its biotin functionality facilitates seamless and specific conjugation to target antibodies or proteins through the streptavidin linkage, enabling precise cell sorting and characterization, thus propelling the fields of immunology and cancer research.
Western Blotting: Enter the realm of Western blotting, where Sulfo-Cyanine5-PEG3-Biotin shines as an extraordinarily sensitive fluorescent marker for detecting biotinylated proteins. Harnessing the biotin-avidin system enhances signal intensity, yielding clear and quantitative results.
Computed Properties | |
|---|---|
| Hydrogen Bond Donor Count | 4 |
| Hydrogen Bond Acceptor Count | 13 |
| Rotatable Bond Count | 23 |
| Exact Mass | 1036.35105192 g/mol |
| Monoisotopic Mass | 1036.35105192 g/mol |
| Topological Polar Surface Area | 281Ų |
| Heavy Atom Count | 69 |
| Formal Charge | 0 |
| Complexity | 2090 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 3 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 3 |
| Covalently-Bonded Unit Count | 2 |
| Compound Is Canonicalized | Yes |
Applications of Fluorescent Probes & Dyes
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