Sulfo-Cyanine3 amine | CAS 2183440-43-7

Sulfo-Cyanine3 amine is a water-soluble cyanine dye featuring an amine-reactive handle for fluorescent labeling workflows. Its sulfonated structure supports aqueous conjugation and imaging compatibility, while the cyanine emission profile makes it useful for fluorescence microscopy and fluorescence-based readouts in labeling, tracking, and assay development. In practice, the reactive amine functionality enables preparation of fluorescent conjugates for biomolecule staining and probe construction.

1. Protein And Antibody Labeling

Sulfo-Cyanine3 amine is used by researchers to generate cyanine-labeled proteins and antibody conjugates for fluorescence imaging and fluorescence assay development. The amine-reactive functionality supports coupling to primary amine groups on lysine-containing biomolecules, enabling preparation of labeled reagents for binding studies, immunofluorescence workflows, and fluorescence-based quantification of biomolecular interactions. Because the dye is sulfonated, conjugates are often handled in aqueous buffers commonly used for labeling and downstream assay steps.

2. Fluorescence Microscopy Staining

Sulfo-Cyanine3 amine is frequently selected for fluorescence microscopy staining and cellular imaging reagent development where a bright red-emitting cyanine reporter is desired. In typical workflows, the dye is conjugated to targeting or affinity reagents (such as proteins or other amine-bearing biomolecules) to visualize binding and localization patterns on prepared samples. The aqueous character of the dye helps streamline conjugate preparation and use in microscopy buffer systems, supporting consistent staining and imaging across experimental sets.

3. Flow Cytometry Fluorescent Conjugates

Sulfo-Cyanine3 amine is applied in flow cytometry workflows that rely on stable fluorescent conjugates for labeling biomolecules and tracking binding events in suspension. Conjugated reagents prepared from Sulfo-Cyanine3 amine are used to tag amine-containing antibodies or proteins, enabling fluorescence readout of reagent binding to cells or biomolecular targets in assay development and analytical characterization. The dye's sulfonated, water-compatible nature supports preparation of conjugates for routine instrument workflows where aqueous staining buffers are standard.

4. Fluorescent Probe Construction

Sulfo-Cyanine3 amine is also used as a building-block dye for constructing fluorescent probes and labeled assay reagents, including secondary labeling reagents and multicomponent imaging tools. By attaching the dye to amine-bearing components, teams can create custom fluorescent conjugates for multiplex visualization strategies and fluorescence-based monitoring in biochemical workflows. This makes Sulfo-Cyanine3 amine a practical choice for laboratories developing in-house fluorescent reagents for microscopy, imaging assays, and fluorescence detection platforms.