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diSulfo-Cy3 alkyne | CAS 2055138-88-8
| Catalog Number | F03-0026 |
| Category | sulfo-Cyanine3 |
| Molecular Formula | C33H38N3NaO7S2 |
| Molecular Weight | 675.8 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
diSulfo-Cy3 azide is a water-soluble dye containing an alkyne group, which enables Click Chemistry. Absorbance and emission of the dye are identical to Cy3 fluorophore. The reagent is designed for the labeling of proteins, and even intact biological objects in water phase.
Chemical Information
Product Specification
Application
| Purity | 95% |
| Solubility | Water, DMSO, DMF, DCM |
| Fluorescence Quantum Yield | 0.15 |
| Excitation | 548 |
| Emission | 567 |
| Storage | -20 °C |
DiSulfo-Cy3 alkyne is a water-soluble fluorescent dye that belongs to the cyanine family, widely recognized for its strong fluorescence and high water solubility. This alkyne derivative is specifically designed for click chemistry applications, allowing it to be conjugated to a variety of molecules through copper-catalyzed azide-alkyne cycloaddition (CuAAC). The sulfonate groups in diSulfo-Cy3 alkyne enhance its solubility in aqueous environments, making it exceptionally suitable for biological and biomedical research. Its fluorescence properties make it an excellent choice for imaging applications, where high sensitivity and precise localization are required.
One of the key applications of diSulfo-Cy3 alkyne is in bioimaging. The dye’s strong fluorescence allows researchers to visualize biological processes in real-time, providing insights into cellular functions and molecular interactions. This is particularly useful in microscopy, where the dye’s high photostability and brightness enable long-term observation of live cells without significant photobleaching, aiding in studies of cell division, protein localization, and intracellular trafficking.
DiSulfo-Cy3 alkyne is also extensively used in flow cytometry, a technique that analyzes the physical and chemical characteristics of particles in a fluid as they pass through at least one laser. In this application, the dye helps label antibodies or other specific molecules, allowing researchers to identify and quantify various cell populations in a sample. Its high water solubility ensures minimal aggregation of the dye-conjugated antibodies, providing accurate and reliable results.
In molecular diagnostics, diSulfo-Cy3 alkyne aids in the development of sensitive assays for detecting various biomolecules. The dye can be incorporated into probes and primers used in techniques such as PCR or hybridization assays, enabling the detection of specific DNA or RNA sequences. This application is crucial for identifying genetic diseases, monitoring disease progression, and validating the efficacy of treatments. The bright fluorescence signal produced by diSulfo-Cy3 improves the sensitivity and specificity of these diagnostic tests.
Another significant application of diSulfo-Cy3 alkyne is in the field of drug discovery and development. Researchers can use this dye to track the distribution and localization of drugs within biological systems, helping to elucidate their mechanisms of action and optimize their delivery. By conjugating diSulfo-Cy3 to pharmacological compounds, scientists are able to monitor drug interactions at the cellular or molecular level, providing valuable data that can guide the development of more effective therapeutic agents.
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