TAMRA NHS ester, 5-isomer | CAS 321862-17-3

TAMRA NHS ester, 5-isomer is a fluorescent NHS-ester (N-hydroxysuccinimide ester) designed for amine-reactive bioconjugation via widely used click-compatible labeling workflows. As a rhodamine-based dye, it provides strong visible fluorescence for tracking biomolecules, surfaces, and materials after covalent attachment to primary amines such as lysine residues and N-termini. The 5-isomer form is commonly selected in labeling pipelines where consistent dye performance and spectral behavior are important for imaging, assay development, and reagent characterization.

1. Antibody And Protein Labeling

TAMRA NHS ester, 5-isomer is routinely employed to generate fluorescently labeled antibodies, enzymes, and protein probes by covalently coupling to accessible lysine side chains and N-terminal amines. Researchers use this reagent to prepare binding reagents for flow cytometry, fluorescence microscopy, and protein localization studies, where stable dye attachment helps maintain signal during washing and incubation steps. Because the NHS-ester reacts under standard aqueous labeling conditions, it fits seamlessly into routine protein conjugation workflows used in chemical biology and molecular imaging labs.

2. Peptide And Biomolecule Tagging

TAMRA NHS ester, 5-isomer is well suited for labeling peptides, oligonucleotide-associated peptides, and other amine-containing biomolecules to create fluorescent substrates and tracking tools. In chemical biology, it is commonly used to introduce a bright, spectrally distinct fluorophore for monitoring binding, uptake, or trafficking in experimental assay formats that rely on covalent dye attachment rather than passive adsorption. The dye’s NHS-ester functionality also supports straightforward incorporation into multi-component labeling strategies where the fluorescent tag must remain stable across buffer exchanges and downstream handling.

3. Surface And Material Functionalization

TAMRA NHS ester, 5-isomer is frequently used to fluorescently functionalize biomaterials and solid supports that present reactive amines, including amine-terminated surfaces, polymer coatings, and porous matrices used in imaging and materials characterization. By enabling covalent attachment of the dye to surface-bound amine groups, the reagent helps researchers visualize coating uniformity, quantify surface labeling, and generate fluorescent readouts for material assembly and stability studies. This application is particularly common in biomaterials science where fluorescent surface markers are used to validate fabrication steps and to support microscopy-based characterization.

4. Diagnostic Reagent Development

TAMRA NHS ester, 5-isomer is commonly incorporated into research diagnostic reagent platforms that require fluorescent labeling of capture molecules, affinity reagents, or assay components. Labeling with an NHS-ester facilitates covalent conjugation to amine-bearing biomolecules used in immunoassays, binding assays, and multiplex fluorescence workflows, supporting robust signal generation and reproducible reagent behavior across assay runs. In diagnostic reagent development, the dye is also used to create reference standards and labeled controls for assay optimization and reagent verification in fluorescence-based detection formats.