BDP 581/591 NHS ester | CAS 654651-21-5

BDP 581/591 NHS ester is a reactive, NHS-ester-functionalized BODIPY dye designed for covalent fluorescent labeling of primary amines. Its bright orange-red fluorescence (581/591 nm emission region) makes it a common choice for building fluorescent conjugates for microscopy and quantitative fluorescence workflows, where stable dye attachment to proteins or peptides is required. Because the dye is pre-activated as an N-hydroxysuccinimide ester, it is routinely used to generate amine-reactive labeling reagents for bioconjugation and downstream imaging assays.

1. Protein And Antibody Labeling

BDP 581/591 NHS ester is widely used to fluorescently label proteins, including antibodies, antibody fragments, and affinity reagents, by targeting accessible lysine residues and N-terminal amines. Researchers incorporate the resulting BDP-labeled biomolecules into immunofluorescence staining, binding studies, and imaging experiments where orange-red emission provides separation from common green-channel fluorophores. In practice, the NHS-ester format supports straightforward conjugate preparation for microscopy platforms and fluorescence-based binding readouts, enabling consistent dye loading for reagent development and assay optimization.

2. Peptide and Biomolecule Conjugation

BDP 581/591 NHS ester supports covalent labeling of peptides and other amine-containing biomolecules used as targeting ligands, affinity tags, or assay components. Chemical biology teams often prepare BDP-labeled peptides for receptor-binding studies, cellular uptake experiments, and fluorescence tracking of biomolecular interactions, leveraging the dye's strong emission in the 581/591 region to improve channel compatibility. The NHS-ester reactivity makes it useful when a stable, non-bleaching covalent dye attachment is preferred over passive adsorption or weaker dye conjugation approaches.

3. Fluorescent Labeling For Flow Cytometry

BDP 581/591 NHS ester is used to generate flow cytometry dyes and labeled detection reagents by covalently attaching the fluorophore to amine-bearing proteins used in immunostaining panels. Flow cytometry users value the orange-red spectral window for multiplexing experiments where separation from green and far-red channels is needed, and where consistent labeling chemistry helps reduce variability between conjugate lots. The covalent NHS-ester labeling strategy also supports preparation of stained standards and labeled controls for instrument setup, gating development, and comparative fluorescence measurements across samples.

4. Fluorescent Probe And Assay Reagent Development

BDP 581/591 NHS ester serves as a building block for fluorescent probe construction and fluorescence assay reagent development, particularly when a stable amine-reactive dye is required to tag assay components. In research settings, teams conjugate the dye to proteins, peptides, or other amine-functional reagents to create fluorescent readouts for binding assays, wash-based immunoassays, and microscopy-compatible labeling reagents. The BDP scaffold's strong fluorescence in the 581/591 region supports assay design where robust signal generation from covalently labeled components is important for reproducible experimental workflows.