BDP 558/568 NHS ester | CAS 150173-73-2

BDP 558/568 NHS ester is a reactive fluorescent labeling dye designed for covalent attachment to primary amines on proteins, peptides, and other amine-containing biomolecules via NHS-ester chemistry. Its excitation/emission in the 550-580/570-600 nm range makes it well matched to common green-to-red fluorescence imaging channels, enabling bright labeling for microscopy, flow cytometry, and fluorescence-based assay development. Researchers frequently use it to generate fluorescent conjugates for tracking, quantification, and multicolor experimental workflows where stable red-orange signal is required.

1. Protein And Antibody Labeling

BDP 558/568 NHS ester is widely used to prepare fluorescent protein conjugates by targeting lysine residues and N-termini on antibodies, enzymes, and affinity reagents. In cell biology and chemical biology workflows, labeled antibodies and protein probes support visualization of binding interactions, monitoring of reagent localization, and quantitative readouts in fluorescence assays. The NHS-ester reactivity enables straightforward conjugate preparation for downstream microscopy and plate-based fluorescence measurements, including experiments where amine-targeted labeling is preferred over dye encapsulation or noncovalent staining.

2. Fluorescence Microscopy Staining

BDP 558/568 NHS ester is used as a labeling reagent for fluorescence microscopy when amine-containing targets need covalent dye attachment for cellular or biomaterial imaging. Common use cases include labeling extracellular matrix components, tracking protein adsorption on surfaces, and generating fluorescently tagged biomolecules for imaging studies of cell-associated structures. The dye's red-orange emission supports imaging in standard fluorescence microscope filter sets and helps researchers maintain signal separation from green-channel labels in multicolor experiments.

3. Flow Cytometry Conjugates

BDP 558/568 NHS ester supports the preparation of flow cytometry dyes for labeling proteins and other amine-bearing reagents used in immunophenotyping and binding assays. Researchers generate fluorescent conjugates to quantify target binding by measuring fluorescence intensity in single-cell suspensions, often selecting this dye when a 560-600 nm detection window is advantageous for spectral separation. Covalent labeling helps reduce dye loss during washing steps, supporting reproducible staining across experimental replicates and enabling multicolor panels that combine multiple fluorophores.

4. Fluorescent Bioassay Reagents

BDP 558/568 NHS ester is frequently incorporated into fluorescence assay development where amine-reactive labeling is needed to build detection reagents such as fluorescent tracers, labeled affinity components, or enzyme/ligand conjugates used as signal-generating partners. In research laboratories, the dye is used to construct assay-ready conjugates for binding studies, reagent-based quantification, and fluorescence readouts in microplate formats. Its emission in the red-orange range aligns well with many plate reader configurations, allowing convenient integration into established fluorescence workflows.