BDY TR-X, SE | CAS 197306-80-2

BDY TR-X, SE is a reactive fluorescent dye designed for conjugation workflows that require a stable fluorescent handle for labeling biomolecules and bioconjugates. The "SE" functional group enables efficient attachment to primary amines, supporting preparation of fluorescent conjugates used in fluorescence microscopy, flow cytometry, and fluorescence-based assays. Its red-emitting character makes it practical for multicolor imaging panels where spectral separation from common green channels is needed.

1. Protein And Antibody Labeling

BDY TR-X, SE is frequently used to generate fluorescently labeled proteins, including antibodies and affinity reagents, for downstream imaging and binding studies. Researchers use the amine-reactive dye to prepare conjugates for fluorescence microscopy and plate-based fluorescence assays, where stable covalent labeling helps maintain reagent performance across washing and incubation steps. In multicolor experiments, the red emission is commonly selected to reduce spectral overlap with FITC- or Alexa Fluor 488-type channels.

2. Flow Cytometry Staining

BDY TR-X, SE supports fluorescent labeling of cell-associated targets by covalently tagging amine-containing biomolecules used in cytometry workflows. Flow cytometry users incorporate BDY TR-X, SE-labeled antibodies, streptavidin conjugates, or other affinity reagents to visualize binding to cell-surface markers and to quantify fluorescence intensity distributions in single-cell measurements. The dye's red spectral region is often leveraged to improve panel design when green and orange channels are already occupied.

3. Fluorescent Bioassay Reagent Building

BDY TR-X, SE is used as a labeling reagent to construct fluorescent assay components such as labeled secondary reagents, detection probes, and fluorescent standards for assay development. In fluorescence-based screening and characterization, conjugation to amine-bearing proteins enables consistent signal generation for readouts including endpoint fluorescence and kinetic fluorescence monitoring. This makes the dye useful for assay workflows that require a covalently attached fluorophore rather than adsorption-based staining.

4. Biomaterial And Surface Functionalization

BDY TR-X, SE is applied in materials research to fluorescently functionalize amine-containing surfaces and biomaterial coatings, enabling visualization of immobilized biomolecules and tracking of surface-bound layers. Researchers use the dye to prepare fluorescently marked coatings for microscopy studies of adsorption, immobilization efficiency, and spatial distribution on engineered substrates. The resulting fluorescent surfaces are also used as convenient reference materials when optimizing conjugation conditions for downstream biosensor or interface studies.