TAMRA DBCO, 5-isomer | CAS 1911598-65-6

TAMRA DBCO, 5-isomer is a TAMRA-based fluorescent labeling reagent equipped with a DBCO (dibenzocyclooctyne) cyclooctyne handle for strain-promoted azide-alkyne cycloaddition (SPAAC). This combination enables copper-free click conjugation to azide-bearing biomolecules and materials, producing fluorescent conjugates for microscopy, flow cytometry, and fluorescence-based assays. The TAMRA fluorophore provides bright visible emission that is widely used for tracking labeled targets in chemical biology and materials research workflows.

1. Biomolecule Azide Labeling

TAMRA DBCO, 5-isomer is used by chemical biologists and analytical researchers to fluorescently label azide-functionalized proteins, peptides, and other biomolecules via copper-free click chemistry. After conjugation, the resulting TAMRA-tagged constructs are commonly employed to monitor biomolecule localization, binding interactions, and trafficking in cell-based imaging workflows, as well as to track conjugate formation in biochemical studies. The DBCO click handle supports straightforward post-labeling of azide-bearing targets without introducing copper, which is particularly valuable for sensitive biomolecule preparations.

2. Fluorescence Microscopy Tracing

TAMRA DBCO, 5-isomer supports fluorescence microscopy experiments where azide-tagged biomolecules or biomaterial components need to be visualized with a TAMRA emission readout. Researchers use the reagent to generate fluorescent conjugates that can be incorporated into labeling schemes for cellular imaging, multicomponent staining, and co-localization studies with other fluorophores. Because the click step is typically performed under mild, copper-free conditions, the reagent is frequently selected for workflows that require labeling after biological assembly steps or for samples where metal exposure is undesirable.

3. Flow Cytometry Conjugates

TAMRA DBCO, 5-isomer is applied in flow cytometry workflows to produce TAMRA-labeled conjugates from azide-functionalized ligands, affinity reagents, or engineered biomolecules. In these applications, the reagent enables consistent fluorescent tagging for measuring binding events, uptake, or population-level labeling readouts using standard visible-excitation cytometers. The DBCO functionality is particularly useful when researchers need to couple fluorescence to pre-functionalized targets without relying on copper-catalyzed methods, supporting streamlined assay development for labeling panels and fluorescence controls.

4. Biomaterial Surface Functionalization

TAMRA DBCO, 5-isomer is used in biomaterials and surface chemistry to fluorescently tag azide-functionalized surfaces, hydrogels, and polymer scaffolds. Materials researchers incorporate the reagent into labeling workflows to visualize surface coverage, track material association with biological components, or validate spatial patterning in engineered constructs. The resulting TAMRA fluorescence provides a practical readout for confirming successful surface modification and for following labeled material interactions in microscopy-based characterization experiments.