5-TAMRA Azide inner salt | CAS 825651-66-9

TAMRA azide, 5-isomer, a highly fluorescent dye, finds extensive utilization in molecular biology and biochemical research. Below are four key applications of TAMRA azide, 5-isomer:

Fluorescent Labeling: The versatile TAMRA azide, 5-isomer serves as a prevalent choice for labeling biomolecules like proteins, nucleotides, and peptides in fluorescence microscopy and flow cytometry. Its intense fluorescence capability facilitates the precise visualization of specific molecules amidst intricate biological specimens. This application is fundamental for exploring cellular processes, scrutinizing protein interactions, and determining molecular localization.

Click Chemistry: Standing as a pivotal reagent in click chemistry, TAMRA azide, 5-isomer empowers the bioconjugation of azide-modified molecules to alkyne-containing counterparts. This highly selective and efficient reaction enables the tagging of biomolecules with fluorescent dyes or other impactful functional groups. Researchers leverage this technique to investigate molecular interactions, track biological processes, and develop cutting-edge diagnostic assays.

DNA Sequencing and Probing: This dye integrates seamlessly into oligonucleotides for use in DNA sequencing and probing methodologies. Through the attachment of TAMRA azide, 5-isomer to DNA probes, scientists achieve remarkable sensitivity and specificity in detecting complementary sequences. This capability proves particularly invaluable in applications like fluorescence in situ hybridization (FISH) and real-time PCR where precise genetic material detection is paramount.

Protein Tracking and Quantification: TAMRA azide, 5-isomer emerges as a vital component in protein tracking endeavors, enabling the real-time monitoring of protein dynamics and interactions within living cells. By conjugating the dye to proteins of interest, researchers gain insights into their localization and movements, employing live cell imaging techniques. Moreover, it plays a pivotal role in quantitative assays, facilitating the measurement of protein concentrations and activity levels across diverse biological samples.