Biotin-PEG4-Dde-TAMRA-PEG3-Azide | CAS 2353409-56-8

Biotin-PEG4-Dde-TAMRA-PEG3-Azide is a multifunctional, azide-bearing fluorescent bioconjugation reagent that integrates a TAMRA fluorophore for red fluorescence readout, a Dde-protected handle for orthogonal stepwise coupling, and a biotin moiety for affinity capture. Its PEG spacers support conjugate flexibility and help reduce steric effects during labeling and downstream immobilization. The azide functionality enables click chemistry workflows that connect the reagent to azide-reactive partners for probe construction, surface functionalization, and fluorescent tracking of biomolecular assemblies.

1. Click-Based Biomolecule Labeling

Biotin-PEG4-Dde-TAMRA-PEG3-Azide is used in chemical biology workflows where researchers need to introduce a fluorescent tag and an affinity handle onto proteins, peptides, or other biomolecules via azide-compatible click conjugation. In practice, the TAMRA signal supports fluorescence microscopy and plate-based fluorescence readouts, while the biotin enables streptavidin-based enrichment, pull-down, or immobilization of the resulting labeled conjugates. The PEG linkers help maintain labeling accessibility, which is particularly useful when attaching bulky biomolecules or building multicomponent probe constructs for imaging and assay development.

2. Streptavidin Capture Assays

Biotin-PEG4-Dde-TAMRA-PEG3-Azide supports fluorescence-based detection strategies that rely on biotin-streptavidin affinity to organize labeled reagents at surfaces or on beads. Researchers commonly use it to generate fluorescent biotinylated materials for binding studies, wash-resistant capture formats, and quantification of conjugate formation by fluorescence readout. The TAMRA fluorophore provides a convenient optical reporter for monitoring the presence of the biotin-tagged species after immobilization, enabling workflow-friendly assay development in biochemical characterization and molecular imaging reagent pipelines.

3. Orthogonal Probe Construction

Biotin-PEG4-Dde-TAMRA-PEG3-Azide is frequently incorporated into multistep labeling schemes where an orthogonal functional handle is needed to build defined fluorescent conjugates. The Dde-protected functionality allows stepwise assembly strategies that can be coordinated with other reactive groups present on biomolecules or scaffold materials, enabling researchers to position the TAMRA reporter and biotin handle with greater control over conjugate architecture. This makes the reagent useful for constructing complex fluorescent probes and affinity-tagged reagents used in microscopy, fluorescence-based tracking, and modular probe development for biomaterials and molecular imaging studies.