TAMRA CPG 500, 5-isomer

TAMRA CPG 500, 5-isomer is a TAMRA (carboxytetramethylrhodamine) fluorescent dye derivative presented on controlled-pore glass (CPG) with a defined isomeric form, enabling robust fluorescent tagging during solid-phase workflows. Its rhodamine core provides strong visible fluorescence that is commonly leveraged in oligonucleotide labeling and fluorescence readouts, while the CPG support supports scalable, automated reagent handling for probe and conjugate construction.

1. Oligonucleotide Labeling

TAMRA CPG 500, 5-isomer is widely used for incorporating a TAMRA fluorophore directly into oligonucleotides during solid-phase synthesis workflows. Researchers and assay developers use the CPG-bound dye to generate fluorescently labeled primers, probes, and oligonucleotide standards for fluorescence-based nucleic acid workflows, including hybridization readouts and fluorescence microscopy of nucleic acid localization. The immobilized format helps streamline automated synthesis and downstream purification strategies, which is particularly valuable when preparing probe sets with consistent labeling positions.

2. Fluorescent Probe Construction

TAMRA CPG 500, 5-isomer supports the preparation of fluorescence-tagged oligonucleotide probes used in molecular imaging and analytical assay development. In chemical biology and nucleic acid analysis labs, the TAMRA label is used to track probe uptake, hybridization performance, and spatial distribution in microscopy experiments, as well as to enable fluorescence readout in plate-based formats. The dye's strong emission in the visible range makes it a practical reporter for designing probe constructs where a stable, bright fluorophore signal is needed for routine instrumentation.

3. Molecular Beacon And FRET Probes

TAMRA CPG 500, 5-isomer is also used as a fluorescent component in nucleic-acid-based signal-control architectures, including molecular beacons and FRET-oriented probe designs where TAMRA serves as a donor or reporter dye. In fluorescence assay development, TAMRA-labeled oligonucleotides are configured into conformational or distance-dependent systems that translate target-dependent structural changes into measurable fluorescence responses. This use case benefits from the ability to place the TAMRA label at a defined location within the oligonucleotide during solid-phase assembly.

4. Fluorescent Standards For Assays

TAMRA CPG 500, 5-isomer is frequently employed to generate fluorescent reference materials and labeled oligonucleotide controls for assay qualification and method development. Molecular imaging groups and nucleic acid analysis teams use TAMRA-labeled constructs as calibration and normalization reagents for fluorescence measurements, helping establish consistent signal baselines across experiments and instrument sessions. The CPG-supported dye format supports repeatable preparation of labeled reagents used in routine fluorescence quantification workflows.