TAMRA-C6-TFP ester | CAS 2183472-92-4

TAMRA-C6-TFP ester is a reactive TAMRA-based fluorescent labeling reagent designed for efficient conjugation to primary amines via the TFP ester functionality. This dye is commonly used in fluorescent bioconjugation workflows where robust red/orange emission is needed for microscopy, flow cytometry, and fluorescence-based assays, including labeling of proteins, peptides, and other amine-containing biomolecules.

1. Protein Amine Labeling

TAMRA-C6-TFP ester is frequently used by molecular biology and chemical biology labs to fluorescently label antibodies, enzymes, and other amine-rich proteins for downstream imaging and quantification. Researchers typically incorporate the reagent into labeling workflows for preparing fluorescent conjugates used in binding studies, protein localization experiments, and assay development where a bright, spectrally distinct TAMRA signal is advantageous. The TFP ester reactivity supports practical coupling to lysine residues and N-termini, enabling preparation of dye-protein conjugates for fluorescence microscopy and plate-based fluorescence readouts.

2. Antibody And Probe Conjugates

TAMRA-C6-TFP ester supports the construction of antibody-fluorophore conjugates and other affinity reagent conjugates used in immunofluorescence and targeted fluorescence experiments. Teams developing fluorescent detection reagents for biomolecular recognition often rely on TAMRA's emission to create readable contrast in multi-color staining schemes and to track binding events in imaging or flow cytometry workflows. By providing a convenient amine-reactive handle, the reagent is used to generate labeled probes for characterizing binding interactions, monitoring conjugate performance in assay buffers, and producing consistent fluorescent reagents for routine laboratory use.

3. Peptide and Biomolecule Tagging

TAMRA-C6-TFP ester is also applied for labeling peptides, small biomolecules, and other amine-containing constructs used in chemical biology and molecular imaging research. Investigators use the reagent to attach TAMRA to peptide ligands, affinity tags, and other building blocks that contain accessible primary amines, enabling fluorescent tracking of biomolecule uptake, binding, or distribution in experimental systems. In probe development contexts, TAMRA labeling is commonly used to generate fluorescent standards and conjugates for optimizing assay conditions, validating reagent handling, and comparing labeling outcomes across batches.

4. Fluorescent Standards For Assays

TAMRA-C6-TFP ester is used in fluorescence assay development to prepare dye-labeled reference materials and calibration tools that support consistent signal generation in experimental workflows. Researchers may generate TAMRA-labeled biomolecule conjugates as internal standards for optimizing assay conditions, verifying instrument settings, and monitoring run-to-run variability in fluorescence measurements. This application is particularly relevant when TAMRA emission provides a convenient detection channel for plate readers, imaging systems, or flow cytometry platforms, and when reproducible fluorescent labeling of amine-bearing components is required for assay reproducibility.