TAMRA amine, 5-isomer | CAS 2158336-48-0

TAMRA amine, 5-isomer is a reactive TAMRA-based fluorescent dye supplied in an amine-functional form, enabling straightforward covalent incorporation into biomolecules and labeling reagents. Its bright red/orange fluorescence is widely used in fluorescence microscopy, flow cytometry, and fluorescence-based assays where stable dye conjugates are required. The amine handle supports coupling strategies for preparing fluorescent conjugates for chemical biology and molecular imaging workflows.

1. Protein And Peptide Labeling

TAMRA amine, 5-isomer is frequently used by protein and peptide labeling groups to generate fluorescent conjugates for imaging and assay development. Researchers incorporate the dye into antibodies, affinity ligands, enzyme reagents, or peptide probes to track binding, localization, and reaction readouts in fluorescence microscopy and plate-based fluorescence assays. The amine functionality supports downstream conjugation to activated carboxyl groups on biomolecules or to amine-reactive handles on carrier scaffolds, providing a practical route to TAMRA-tagged bioconjugates used in labeling studies and reagent characterization.

2. Oligonucleotide And Nucleic Acid Probes

TAMRA amine, 5-isomer is used to prepare fluorescent oligonucleotide probes for nucleic acid analysis workflows that rely on red-emitting readouts. Molecular biology laboratories and chemical biology teams attach the dye to nucleic acid scaffolds to visualize hybridization behavior, monitor probe incorporation, or generate fluorescent standards for assay optimization. In these applications, TAMRA's emission in the visible range supports common fluorescence instrumentation used for gel imaging, microplate detection, and fluorescence-based nucleic acid workflows, while the amine handle enables conjugate construction compatible with typical oligonucleotide labeling pipelines.

3. Fluorescent Microscopy Tracing

TAMRA amine, 5-isomer supports cellular and biomaterial tracing experiments where red/orange fluorescence provides clear contrast against common background signals. Imaging teams label targeting peptides, extracellular matrix components, nanoparticles, or other biomolecule carriers with TAMRA to follow distribution, uptake, or co-localization in microscopy studies. The dye's covalent conjugation approach helps maintain signal during washing steps and multi-step staining workflows, making it a practical fluorophore for constructing multi-color staining panels and for quantitative fluorescence imaging of labeled materials in research settings.

4. Flow Cytometry Fluorescent Tagging

TAMRA amine, 5-isomer is used to generate flow cytometry-compatible fluorescent tags for labeling cells or cell-associated reagents in suspension assays. Flow cytometry users conjugate the dye to antibodies, ligands, or labeling reagents to quantify binding and relative fluorescence intensity across experimental conditions. The amine functionality supports reagent preparation for consistent dye loading and conjugate handling, enabling TAMRA-labeled probes to be incorporated into panel designs that require a red-emitting channel for fluorescence gating and analysis.