sulfo-Cyanine3 hydrazide | CAS 2144762-62-7

Sulfo-Cyanine3 hydrazide is a water-soluble cyanine dye hydrazide designed for labeling workflows that target aldehyde-containing biomolecules and materials. The hydrazide functionality enables efficient conjugation to carbonyl groups under common bioconjugation conditions, producing stable dye conjugates that are widely used in fluorescence microscopy, labeling of glycans and oxidized biomolecules, and fluorescence-based tracking. Its cyanine emission in the visible range supports bright detection on standard fluorescence imaging platforms.

1. Glycan And Carbohydrate Labeling

Sulfo-Cyanine3 hydrazide is frequently used to label oxidized carbohydrates, glycoproteins, and glycan-containing biomolecules for downstream fluorescence imaging and analysis. Researchers employ it to introduce a Cy3 fluorophore onto aldehyde-bearing sugars generated from glycan oxidation strategies, enabling visualization of glycan distribution on proteins, cell-surface samples, or purified carbohydrate conjugates. The sulfonate-containing dye structure supports aqueous handling in labeling buffers, which is helpful for preparing fluorescent glycoconjugates for microscopy and fluorescence readouts.

2. Protein And Peptide Fluorescent Labeling

Sulfo-Cyanine3 hydrazide supports fluorescent tagging of proteins and peptides that have been converted to aldehyde-containing forms, enabling tracking of biomolecule localization and interaction in chemical biology workflows. Common use cases include labeling oxidized protein fragments, aldehyde-functionalized peptide conjugates, and carbonyl-containing biomaterials where a Cy3 reporter is needed for visualization. The resulting Cy3 conjugates are used in fluorescence microscopy and fluorescence-based assays to monitor biomolecule presence, distribution, and relative abundance in experimental samples.

3. Biomaterial Surface Functionalization

Sulfo-Cyanine3 hydrazide is applied in materials research to create fluorescently labeled surfaces and coatings by coupling the dye to aldehyde-functionalized polymers, hydrogels, or biomaterial scaffolds. In biomaterials and surface engineering workflows, researchers incorporate the dye to enable optical readouts of coating uniformity, surface retention, or spatial patterning during material characterization. The water-soluble nature of the sulfonated cyanine dye helps streamline labeling steps in aqueous processing environments used for hydrogel and polymer functionalization.

4. Fluorescence Imaging And Tracking

Sulfo-Cyanine3 hydrazide-derived conjugates are used as Cy3 fluorescence tags for cellular and molecular imaging studies where aldehyde-bearing targets are available. Laboratories rely on these conjugates for tracking labeled biomolecules in microscopy experiments, including studies of uptake, binding, and transport in labeled sample preparations. The Cy3 reporter provides a convenient visible-channel signal for standard fluorescence microscopes and plate-based fluorescence workflows, supporting comparative imaging across experimental conditions.