ROX azide, 5-isomer | CAS 2628213-67-0

ROX azide, 5-isomer is a ROX-class azide-functional fluorescent labeling reagent designed for copper-catalyzed azide-alkyne cycloaddition (CuAAC) workflows. As an azide partner, it enables efficient attachment of a red/orange-emitting ROX fluorophore to alkyne-bearing biomolecules, linkers, and polymer or surface platforms. In fluorescence-based molecular labeling and probe construction, the ROX emission is commonly leveraged for multiplex-friendly imaging and assay readouts where red spectral channels are preferred.

1. Biomolecule Labeling

ROX azide, 5-isomer is used by chemical biology and protein chemistry groups to generate fluorescently labeled biomolecules via click conjugation to alkyne-containing proteins, peptides, and affinity reagents. Researchers incorporate the ROX fluorophore into labeling workflows for tracking binding interactions, monitoring biomolecule uptake or trafficking in cell-based studies, and building fluorescent conjugates for downstream assay development. The azide handle supports modular probe construction, allowing users to standardize labeling by reacting ROX azide with a wide range of alkyne-bearing targets (including commercially available alkyne-tagged building blocks).

2. Fluorescent Probe Construction

ROX azide, 5-isomer is frequently selected for constructing ROX-based fluorescent probes and imaging reagents where a red-emitting fluorophore is required for microscopy or plate-reader formats. In probe development, the azide functionality provides a convenient point for installing the fluorophore onto recognition scaffolds such as small-molecule carriers, oligonucleotide conjugates, or polymeric sensing elements that have been functionalized with terminal alkynes. This approach supports rapid generation of probe variants for assay optimization, including experiments where ROX emission is used to separate signals from green or blue channels in multicolor experiments.

3. Surface And Polymer Functionalization

ROX azide, 5-isomer is applied in biomaterials and materials chemistry to fluorescently tag surfaces and polymer networks that present alkyne groups for click attachment. Researchers use the reagent to create ROX-labeled hydrogels, coatings, and functional polymer conjugates that enable visualization of material distribution, surface patterning, and labeling of biomaterial interfaces. This ROX installation strategy is also used in instrument-facing workflows such as fluorescent standards preparation and imaging-based characterization of engineered materials where stable, covalent fluorophore attachment is required.