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5-Carboxy-x-rhodamine triethylammonium salt | CAS 216699-35-3
| Catalog Number | F05-0008 |
| Category | Rhodamine |
| Molecular Formula | C33H30N2O5?C6H15N |
| Molecular Weight | 669.302 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
5-Carboxy-x-rhodamine triethylammonium salt is a fluorochrome dye of immense significance in the field of biomedical research. It finds extensive usage in cutting-edge techniques such as fluorescence microscopy and flow cytometry. Leveraging its remarkable photostability and luminosity, scientists employ this dye for labeling crucial biomolecules, including proteins and nucleic acids, enabling their visualization and comprehensive analysis. This versatile compound presents itself as an invaluable tool for a myriad of applications such as cell imaging, immunohistochemistry, and live-cell tracking.
Chemical Information
Product Specification
Application
| Synonyms | 5-Carboxy-X-rhodamine;5-ROX, CXR |
| Purity | >95.0%(HPLC) |
| IUPAC Name | 3-carboxy-4-(3-oxa-23-aza-9-azoniaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4,9(28),13,15,18-heptaen-16-yl)benzoate |
| Canonical SMILES | C1CC2=CC3=C(C4=C2N(C1)CCC4)OC5=C6CCC[N+]7=C6C(=CC5=C3C8=C(C=C(C=C8)C(=O)[O-])C(=O)O)CCC7 |
| InChI | InChI=1S/C33H30N2O5/c36-32(37)20-9-10-21(24(17-20)33(38)39)27-25-15-18-5-1-11-34-13-3-7-22(28(18)34)30(25)40-31-23-8-4-14-35-12-2-6-19(29(23)35)16-26(27)31/h9-10,15-17H,1-8,11-14H2,(H-,36,37,38,39) |
| InChIKey | UNGMOMJDNDFGJG-UHFFFAOYSA-N |
| Appearance | Solid |
| MDL Number | MFCD28355897 |
| Condition To Avoid | Light Sensitive,Air Sensitive,Moisture Sensitive,Heat Sensitive |
| Storage | -20 °C |
| Store Under Inert Gas | Store under inert gas |
5-Carboxy-x-rhodamine triethylammonium salt, a fluorescent dye renowned for its exceptional photostability and vibrant fluorescence, finds widespread use across diverse scientific domains. Here are four key applications of this remarkable dye：
Fluorescence Microscopy: Revered in the realm of microscopy, this dye serves as a go-to choice for labeling biological specimens, enabling researchers to illuminate cells, tissues, and organelles with unparalleled specificity and resolution. By conjugating antibodies or biomolecules with 5-Carboxy-x-rhodamine, scientists delve into the intricate realm of cellular processes, protein localization, and tissue architecture.
Flow Cytometry: Embraced in the field of flow cytometry, 5-Carboxy-x-rhodamine triethylammonium salt emerges as a pivotal tool for staining cells, facilitating the nuanced analysis of diverse cell populations based on their fluorescence signatures. Through conjugation with antibodies targeting specific cell surface markers, this dye empowers researchers to swiftly and quantitatively assess cell types, protein expression levels, and functional statuses within complex cell mixtures, shedding light on cellular dynamics with clarity and depth.
Real-Time PCR: In the intricate landscape of molecular biology, 5-Carboxy-x-rhodamine emerges as a beacon of innovation, serving as a fluorescent reporter dye in quantitative real-time PCR (qPCR) assays. As it fluoresces upon DNA probe hybridization, this dye enables real-time monitoring of DNA amplification, ensuring precise and sensitive quantification of nucleic acids in diverse samples.
Protein and Nucleic Acid Labeling: Beyond its conventional uses in microscopy and flow cytometry, 5-Carboxy-x-rhodamine takes center stage in labeling proteins and nucleic acids for a myriad of biochemical assays. By conjugating with oligonucleotides, peptides, and various biomolecules, this dye unlocks the realm of molecular interactions, kinetics, and biomolecular dynamics, facilitating the detection and tracking of key players in experimental systems.
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