R110 azide, 6- isomer | CAS 1622395-29-2

R110 azide, 6- isomer is an azide-functionalized rhodamine-derived fluorescent dye designed for copper-free click chemistry workflows that enable efficient attachment to biomolecules and materials. Its rhodamine-like fluorescence supports bright labeling in common fluorescence microscopy and plate-reader formats, while the azide handle provides a practical reactive site for downstream conjugation strategies. Researchers use this reagent to build fluorescent conjugates, construct labeling reagents, and generate traceable probes for chemical biology and imaging assays.

1. Click-Based Biomolecule Labeling

R110 azide, 6- isomer is used as a fluorescent labeling handle in copper-free click conjugation workflows to attach a rhodamine-type fluorophore to proteins, peptides, and other biomolecular scaffolds that have been pre-functionalized with complementary cyclooctyne or strained-alkyne groups. Chemical biology groups rely on this approach to generate fluorescent conjugates for tracking biomolecule distribution, monitoring binding interactions, and visualizing labeled targets in microscopy-based experiments. The azide functionality allows modular probe assembly, supporting rapid swapping of targeting ligands while keeping the same fluorophore reporting unit.

2. Fluorescence Microscopy Tracing

R110 azide, 6- isomer is commonly incorporated into fluorescent imaging reagents after conjugation to cell-associated or biomaterial-associated components, enabling visualization of labeled structures and chemical tags in fluorescence microscopy. Imaging teams use the rhodamine-like emission behavior to follow conjugate localization within experimental samples, including labeled biomolecule assemblies and fluorescently tagged surfaces used in cell culture and materials studies. This reagent is particularly useful when the labeling strategy benefits from a bioorthogonal "add the dye at the end" workflow, reducing perturbation from dye exposure during earlier steps.

3. Fluorescent Probe Construction

R110 azide, 6- isomer serves as a fluorophore building block for constructing fluorescent probes and reporter conjugates in assay development pipelines. Probe developers use the azide group to integrate the dye into larger sensing or reporting constructs, such as fluorescently tagged affinity reagents, multicomponent imaging probes, and fluorescent standards for workflow validation. In these applications, the dye's strong visible fluorescence readout supports straightforward detection using standard fluorescence instrumentation, while the click-compatible handle enables flexible synthesis of probe libraries for comparative studies.

4. Biomaterial Surface Functionalization

R110 azide, 6- isomer is applied in biomaterials research to introduce fluorescent tags onto polymer surfaces, hydrogels, and other functional materials via click-based attachment to complementary reactive groups. Materials scientists use fluorescently labeled surfaces to study adsorption, coating uniformity, and spatial organization of biomolecular layers under experimental conditions. The azide handle supports modular surface engineering, allowing researchers to tune labeling density and combine the fluorophore with other functional motifs used for cell-interaction studies and imaging-guided material characterization.