ROX alkyne, 5-isomer | CAS 2264016-88-6

ROX alkyne, 5-isomer is a ROX-based fluorescent alkyne designed for click chemistry labeling workflows where a ROX emission readout is required. As a terminal alkyne handle, it is commonly used to introduce a bright red/orange fluorophore into biomolecules, probes, and materials via copper-catalyzed azide-alkyne cycloaddition (CuAAC) or related click formats. The ROX fluorophore enables fluorescence detection in standard fluorescence imaging and plate-reader assays that monitor red-shifted emission.

1. Fluorescent Biomolecule Labeling

ROX alkyne, 5-isomer is frequently used by chemical biology and proteomics groups to generate ROX-labeled proteins, peptides, and other biomolecular conjugates using azide-functional partners. Researchers incorporate the alkyne handle into their labeling strategy to prepare fluorescent tracking reagents for binding studies, wash-and-detect workflows, and comparative imaging experiments where a ROX-compatible detection channel is available. This reagent is also used when investigators want to standardize labeling across multiple targets by starting from a consistent ROX fluorophore platform and coupling it to azide-bearing biomolecules.

2. Fluorescent Probe Construction

ROX alkyne, 5-isomer supports fluorescent probe development in assay and imaging reagent pipelines that rely on click-compatible modular assembly. Teams building labeled affinity probes, imaging reagents, or multicomponent fluorescence tools often use the terminal alkyne to attach ROX to azide-functional scaffolds, such as probe backbones, targeting ligands, or assay components. The resulting ROX-tagged constructs are then used in fluorescence microscopy and plate-based fluorescence assays to visualize localization, monitor binding events, or provide a stable fluorescent reporter for downstream readouts.

3. Biomaterial And Surface Functionalization

ROX alkyne, 5-isomer is applied in biomaterials science and materials chemistry to create ROX-labeled surfaces and polymer-modified platforms through click conjugation to azide-functional materials. Researchers use these ROX-tagged coatings and functional layers as fluorescent markers for surface characterization, microscopy-based mapping of material distribution, and validation of functionalization steps in hydrogel or polymer systems. In workflows that require spatially resolved fluorescence readouts, the ROX alkyne handle enables straightforward incorporation of a red/orange fluorescent reporter into otherwise non-fluorescent materials.

4. Nucleic Acid Labeling Workflows

ROX alkyne, 5-isomer is also used in nucleic acid analysis development when a ROX fluorescent tag is required and the labeling partner can be presented with an azide functionality. Molecular biology teams employ click chemistry labeling to generate ROX-labeled oligonucleotides for fluorescence-based detection formats, including hybridization readouts and labeled probe constructs used in nucleic acid workflows. This approach is particularly useful when researchers want to attach a ROX reporter to an oligonucleotide scaffold using a modular, handle-based conjugation strategy compatible with standard fluorescence instrumentation.