3,6,9,12,15,18,21,24,27-Nonaoxatriacont-29-yn-1-ol | CAS 2055022-35-8

3,6,9,12,15,18,21,24,27-Nonaoxatriacont-29-yn-1-ol is a long, highly polyether-based alkyne-bearing click chemistry reagent designed for bioorthogonal conjugation workflows. As a terminal propargyl alcohol/alkyne functionality embedded in a polyethylene glycol-like scaffold, it is well suited to copper-free and copper-mediated click labeling strategies that install alkyne handles into biomolecules, surfaces, and polymeric materials. Its extended hydrophilic chain architecture is commonly leveraged to reduce nonspecific interactions, improve solubility, and enable robust downstream conjugate assembly for research-grade chemical biology and materials applications.

1. PEGylated Probe Labeling

3,6,9,12,15,18,21,24,27-Nonaoxatriacont-29-yn-1-ol is used as an alkyne handle for constructing PEG-like, hydrophilic labeling reagents for chemical biology tool development. Researchers incorporate the reagent into probe scaffolds to create conjugates that tolerate aqueous handling and minimize aggregation during imaging and assay development. The terminal propargyl alcohol functionality supports click-based attachment to azide-bearing targets, enabling modular assembly of fluorescent, affinity, or activity-reporting probes. The long polyether segment is particularly valuable when attaching labels to proteins, peptides, or carbohydrate-rich constructs where steric accessibility and background binding are key considerations.

2. Surface And Material Functionalization

3,6,9,12,15,18,21,24,27-Nonaoxatriacont-29-yn-1-ol is applied to functionalize polymeric and biomaterial surfaces with an alkyne moiety for subsequent click coupling to azide-modified ligands. In materials science workflows, the reagent is commonly used to introduce antifouling, hydration-enhancing spacers while preserving reactive click chemistry at the interface. This approach supports the creation of patterned or uniformly functional coatings for biosensing platforms, cell-interaction studies, and microfabricated assay surfaces where controlled presentation of capture elements is required. The polyether chain helps maintain accessibility of the clickable group and can improve stability of the functional layer under repeated washing and buffer exposure.

3. Biomolecule Conjugation Platforms

3,6,9,12,15,18,21,24,27-Nonaoxatriacont-29-yn-1-ol serves as a versatile building block for generating alkyne-functional biomolecule conjugation platforms. Bioconjugation teams use it to install click-ready handles on biomolecular carriers, such as protein scaffolds, antibody fragments, enzyme conjugates, and nucleic-acid-associated constructs, typically through linker strategies that preserve the terminal alkyne for downstream coupling. The extended hydrophilic backbone is often selected to improve conjugate solubility and reduce nonspecific adsorption, which is advantageous during labeling, purification, and storage. Such platforms are frequently adopted in research reagent development where modularity and reproducibility across multiple labeling targets are important.

4. Multivalent Imaging Reagent Design

3,6,9,12,15,18,21,24,27-Nonaoxatriacont-29-yn-1-ol is used to design multivalent imaging and detection reagents that benefit from spatially separated functional groups. The reagent’s long polyether chain provides a flexible spacer that can reduce steric crowding when multiple click couplings are performed to assemble complex probe architectures. In molecular imaging and diagnostic reagent development, teams often rely on this type of hydrophilic alkyne handle to improve probe behavior in aqueous buffers and to support consistent conjugation density. Modular attachment to azide-bearing reporters enables rapid iteration of probe formats for method development, reagent optimization, and comparative studies across different labeling chemistries.

5. Click-Ready Linker For Nanoconjugates

3,6,9,12,15,18,21,24,27-Nonaoxatriacont-29-yn-1-ol is incorporated into linker systems used to prepare click-ready nanoconjugates and nanoparticle-associated reagents. Nanomaterials groups use the reagent to introduce an alkyne functionality on particle coatings, polymer shells, or surface-grafted layers while maintaining colloidal stability through the hydrophilic polyether environment. Subsequent click coupling to azide-functional ligands supports assembly of multicomponent constructs, including affinity-tagged, receptor-targeting, or reporter-decorated nanomaterials used as research tools. The scaffold’s spacer properties are particularly relevant when tuning the distance between the nanoparticle surface and the attached functional moieties to achieve reliable presentation in complex media.