Carboxyrhodamine 110-PEG3-Azide | CAS 1536327-95-3

Carboxyrhodamine 110-PEG3-Azide is a rhodamine 110-derived fluorescent labeling reagent bearing a PEG3 linker and a terminal azide handle for copper-free click conjugation workflows. The carboxyl group enables downstream attachment to amine-bearing biomolecules or surfaces after standard coupling chemistry, while the azide functionality supports modular construction of fluorescent conjugates, probes, and labeled materials. Its rhodamine 110 fluorescence is commonly leveraged for sensitive fluorescence readouts in labeling, imaging, and assay development where a reactive azide tag is advantageous.

1. Fluorescent Bioconjugation

Carboxyrhodamine 110-PEG3-Azide is used in fluorescence bioconjugation to build labeled proteins, peptides, and other biomolecules through azide-based click coupling to complementary alkyne-bearing partners. Researchers in chemical biology and molecular imaging workflows rely on the PEG3 spacer to reduce steric effects during conjugate assembly and to help maintain labeling accessibility on large biomolecular scaffolds. The carboxyl functionality further supports preparation of fluorescent conjugates for downstream immobilization or for coupling into custom probe formats used in fluorescence imaging and fluorescence-based assays.

2. Probe Construction For Imaging

Carboxyrhodamine 110-PEG3-Azide serves as a modular fluorophore building block for fluorescent probe development, particularly when a reactive azide tag is required for late-stage conjugation. Probe developers use the reagent to generate imaging reagents where the rhodamine 110 signal provides a bright fluorescence readout compatible with standard fluorescence microscopy setups. In practice, the PEG3-azide design supports rapid assembly of multi-component probes, including polymer- or nanoparticle-associated fluorescent constructs, where the fluorophore must be attached reproducibly to a defined targeting or scaffold element.

3. Surface And Material Labeling

Carboxyrhodamine 110-PEG3-Azide is commonly incorporated into biomaterials and surface functionalization strategies to create fluorescently traceable materials and coatings. Materials scientists and biomaterials researchers use the azide handle to attach the fluorophore to alkyne-functional surfaces or to label polymeric systems that are engineered for fluorescence tracking. The carboxyl group also supports preparation of fluorescently labeled materials that can be coupled into immobilized platforms used for microscopy-based characterization, material tracking, and fluorescence readout in assay-adjacent materials studies.