R6G phosphoramidite, 6-isomer | CAS 1355330-47-0

R6G phosphoramidite, 6-isomer is a rhodamine 6G-derived fluorescent building block provided in a phosphoramidite format, enabling efficient incorporation into oligonucleotides for fluorescent labeling and probe construction. Its rhodamine fluorophore core supports bright visible fluorescence for fluorescence imaging and assay development, while the phosphoramidite handle is designed for nucleic acid conjugation workflows. Researchers use this reagent to generate defined, site-specific fluorescent oligo conjugates for microscopy and fluorescence-based analytical assays.

1. Oligonucleotide Fluorescent Labeling

R6G phosphoramidite, 6-isomer is used by molecular biology and chemical biology teams to install a rhodamine 6G fluorophore at a defined position within DNA or RNA during oligonucleotide synthesis. This enables construction of fluorescently tagged primers, probes, and standards used in nucleic acid workflows where controlled dye placement and consistent conjugate structure are important. Common downstream uses include fluorescence-based readouts for nucleic acid binding studies, hybridization experiments, and labeled oligo controls for assay optimization.

2. Fluorescence Microscopy Tracing

R6G phosphoramidite, 6-isomer supports cellular and molecular imaging workflows that rely on fluorescent nucleic acid tracers. By producing fluorescent oligonucleotides with a rhodamine 6G signal, researchers can visualize labeled nucleic acid behavior in microscopy experiments such as uptake/trafficking studies, localization tracking of nucleic acid probes, and fluorescence-guided sample characterization. The phosphoramidite format is particularly valuable when a defined dye position on the oligo is required for reproducible imaging performance across experimental batches.

3. FRET Probe Oligos

R6G phosphoramidite, 6-isomer is frequently incorporated into oligonucleotide-based FRET systems where rhodamine emission serves as a donor or acceptor component within a nucleic acid context. Teams developing fluorescence resonance energy transfer probes use dye-labeled oligos to monitor hybridization-dependent proximity changes, conformational shifts, or target-driven assembly of fluorescent components. This reagent is especially useful for building FRET probe architectures that require precise fluorophore installation on the same synthetic platform as the recognition sequence.

4. Fluorescent Nucleic Acid Standards

R6G phosphoramidite, 6-isomer is also used to prepare fluorescent oligonucleotide standards and calibration reagents for fluorescence quantification in nucleic acid analysis. Researchers employ dye-labeled oligos as reference materials for instrument setup, assay normalization, and method development in fluorescence-based nucleic acid assays. Because the dye is introduced through the oligo synthesis workflow, the resulting conjugates provide a consistent fluorescent reporter for comparing signal across experiments and batches.