Alkyne-PEG4-phosphoramidite | CAS 1682657-14-2

Alkyne-PEG4-phosphoramidite is a click-chemistry-enabled phosphoramidite building block designed for incorporation of an alkyne handle into nucleic-acid materials and related conjugation platforms. As a reagent used to introduce a terminal alkyne functionality, it supports copper-catalyzed azide–alkyne cycloaddition (CuAAC) workflows that are widely used for modular labeling, surface patterning, and multistep probe assembly. The PEG4 spacer embedded in the construct helps reduce steric constraints and improves accessibility of the alkyne for downstream conjugation, making it a practical choice for research-grade oligonucleotide conjugates and PEG-mediated biomolecular interfaces.

1. Oligonucleotide Click Labeling

Alkyne-PEG4-phosphoramidite is commonly used to functionalize DNA or RNA oligonucleotides with a terminal alkyne for subsequent CuAAC coupling to azide-bearing dyes, affinity tags, or reporter groups. Researchers use the PEG4 spacer to maintain efficient access to the reactive alkyne even when the oligonucleotide is folded, hybridized, or immobilized, enabling consistent labeling density across different probe formats. This approach is frequently adopted in molecular biology and chemical biology workflows where modular post-synthetic conjugation is preferred over direct dye incorporation during oligonucleotide synthesis.

2. Bioconjugation Linker Platforms

Alkyne-PEG4-phosphoramidite is used as a convenient handle to generate click-ready oligonucleotide conjugates and hybrid biomolecular constructs that can be linked to azide-functional partners. In chemical biology and biomaterials research, the alkyne introduced via the phosphoramidite format supports iterative assembly of multicomponent systems, such as oligonucleotide-based scaffolds decorated with multiple functional moieties. The PEG4 element is particularly valuable when conjugation partners are bulky or when the conjugate must remain soluble and accessible for binding assays, imaging workflows, or analytical readouts in lab settings.

3. Surface Immobilization And Patterning

Alkyne-PEG4-phosphoramidite is applied to prepare immobilizable nucleic-acid layers and patterned surfaces where alkyne-bearing oligonucleotides act as defined attachment points for azide-functional materials. Materials scientists and diagnostic reagent developers use this strategy to build spatially controlled probe arrays, capture surfaces, and modular sensor interfaces by coupling the alkyne-functional strands to azide-derivatized linkers, nanoparticles, or polymer components. The PEG4 spacer helps mitigate steric crowding at the interface, supporting reliable attachment and improved accessibility of the conjugated features after immobilization.

4. Molecular Imaging Probe Assembly

Alkyne-PEG4-phosphoramidite is frequently incorporated into imaging probe oligonucleotides and related molecular tracers to enable late-stage installation of azide-functional fluorophores or other imaging reporters via CuAAC. Chemical biology groups value the modularity because the imaging label can be selected and optimized after the oligonucleotide is synthesized, purified, and characterized. The PEG4 spacer supports efficient coupling and reduces steric hindrance around the reactive site, which is important for maintaining strong labeling performance in probe libraries and multicolor imaging reagent development workflows.