Propargyl-PEG4-tetra-Ac-beta-D-glucose | CAS 1360446-31-6

Propargyl-PEG4-tetra-Ac-beta-D-glucose is a PEGylated, multivalent carbohydrate click chemistry reagent built around a propargyl handle for copper-catalyzed azide–alkyne cycloaddition (CuAAC) and an acetyl-protected beta-D-glucose motif presented in a tetra-configuration. The PEG4 spacer and the clustered sugar architecture are designed to improve aqueous compatibility and enable multivalent binding to lectins and carbohydrate-recognizing biomolecules. This reagent is commonly used as a functional glycan building block for generating azide-functional glycoprobes, glyco-materials, and imaging/assay platforms where controlled presentation of glucose on surfaces or macromolecular scaffolds is required.

1. Lectin Binding Probes

Propargyl-PEG4-tetra-Ac-beta-D-glucose is widely used to construct multivalent glucose ligands for lectin recognition studies, where the tetra-acetylated beta-D-glucose arrangement provides dense carbohydrate display that is well suited to lectin/lectin-like receptor binding assays. Researchers incorporate the propargyl group to attach the glycan motif to azide-bearing fluorescent dyes, affinity tags, or carrier proteins, enabling systematic evaluation of how linker length, valency, and glycan density influence binding behavior. The PEG4 spacer supports dispersion in aqueous assay buffers and helps maintain accessibility of the carbohydrate ligands during probe preparation and downstream characterization.

2. Glycoprotein And Surface Labeling

Propargyl-PEG4-tetra-Ac-beta-D-glucose serves as a practical glycan-labeling reagent for decorating azide-functional biomaterials and bioconjugates with glucose-rich surfaces. In glycoengineering workflows, the propargyl handle enables efficient CuAAC coupling to azide groups introduced on proteins, peptides, nanoparticles, or polymer coatings, allowing researchers to generate glycoprotein mimics and carbohydrate-functional surfaces for binding and interaction mapping. The PEG4-tethered, multivalent sugar presentation is particularly useful when the goal is to create stable, water-compatible glycan interfaces for analytical assays, receptor screening toolkits, and surface-sensitive studies.

3. Molecular Imaging And Fluorescent Tags

Propargyl-PEG4-tetra-Ac-beta-D-glucose is used as a clickable carbohydrate module to prepare fluorescent or imaging-compatible glycoprobes that report on carbohydrate recognition events in cell-free and biochemical imaging formats. By coupling the propargyl group to azide-functional imaging reporters, investigators can generate multivalent glucose probes with improved solubility and reduced aggregation compared with non-PEGylated analogs. The tetra-glucose architecture supports strong signal generation in imaging workflows that rely on multivalent interactions, while the CuAAC-compatible handle streamlines probe assembly for microscopy, flow-based readouts, and imaging reagent libraries.

4. Glyco-Materials For Diagnostics Research

Propargyl-PEG4-tetra-Ac-beta-D-glucose is commonly integrated into diagnostic-research materials where carbohydrate-functional surfaces are needed to capture or interrogate lectin-like targets and carbohydrate-binding proteins. Materials scientists use the propargyl functionality to click the reagent onto azide-derivatized hydrogels, membranes, and polymer particles, producing glucose-presenting platforms that can be incorporated into assay development pipelines and binding-interaction characterization. The PEG4 spacer and multivalent sugar display help create reproducible glycan densities and water-compatible interfaces, supporting robust fabrication of glyco-biomaterial components used in reagent development and analytical method optimization.