Propargyl-PEG13-acid | CAS 1421676-62-1

Propargyl-PEG13-acid is a PEG-based alkyne-functionalized linker designed for copper-free or copper-catalyzed click chemistry workflows, most commonly used in strain-promoted or azide–alkyne cycloaddition contexts depending on the complementary partner. The long, hydrophilic PEG spacer improves aqueous compatibility and spacing control for bioconjugation, while the terminal propargyl group provides a reliable handle for attaching biomolecules, surfaces, or imaging labels. This reagent is frequently selected in molecular imaging, diagnostic reagent development, and biomaterials functionalization where stable conjugation chemistry and water-soluble architectures are required.

1. Protein And Peptide Labeling

Propargyl-PEG13-acid is widely used to introduce an alkyne functionality into protein and peptide conjugation pipelines, enabling downstream attachment of azide-bearing reporters, affinity tags, or capture moieties through click-compatible coupling. The PEG13 spacer helps reduce steric congestion and nonspecific interactions, which is particularly valuable when preparing multivalent labeling reagents for analytical workflows such as Western blotting, immunoassays, and targeted binding studies. Researchers often pair Propargyl-PEG13-acid with azide-functional dyes, biotin analogs, or polymeric tags to generate well-defined conjugates for detection and characterization in solution or on solid supports.

2. Surface and Material Functionalization

Propargyl-PEG13-acid supports alkyne-bearing surface modification strategies for biomaterials and diagnostic platforms where controlled presentation of functional ligands is critical. The PEG linker can be used to build antifouling or spacing layers that improve accessibility of reactive groups on nanoparticles, hydrogel matrices, and sensor surfaces, while the terminal alkyne provides a click handle for subsequent immobilization of azide-functional components. This approach is commonly adopted in materials science and chemical biology labs to create reusable binding surfaces, patterned materials, and modular platforms for attaching affinity ligands, imaging probes, or assay reagents with minimal impact from harsh coupling conditions.

3. Molecular Imaging Probe Assembly

Propargyl-PEG13-acid is a practical building block for assembling imaging probes and tracking reagents that require water-soluble linkers and reliable click-compatible attachment points. By incorporating the propargyl group into probe scaffolds, teams can efficiently connect azide-functional fluorophores, luminescent reporters, or other imaging tags while leveraging the PEG13 chain to maintain solubility and reduce aggregation. In molecular imaging reagent development, Propargyl-PEG13-acid is often chosen to tune linker length and hydrophilicity so that labeling does not overly restrict the probe’s interaction with its targeting or binding partner, supporting robust probe handling during formulation and assay preparation.

4. Diagnostic Reagent Conjugation

Propargyl-PEG13-acid is frequently incorporated into diagnostic reagent development workflows to create modular conjugates that can be assembled from separately prepared azide and alkyne components. The PEG13 acid-containing functionality and the terminal alkyne enable construction of stable, well-spaced conjugates for assay formats that rely on multicomponent assembly, including affinity-based detection reagents and labeling reagents used in high-throughput analytical studies. Teams in chemical biology and translational research support functions commonly use Propargyl-PEG13-acid to standardize linker chemistry across reagent lots, improving reproducibility of conjugate assembly for downstream assay development and characterization.