NH-bis(PEG2-propargyl) | CAS 2100306-83-8

NH-bis(PEG2-propargyl) is a PEG-based bifunctional click chemistry reagent bearing two terminal propargyl groups, designed for copper-catalyzed azide–alkyne cycloaddition (CuAAC) and related alkyne-driven conjugation workflows. Its flexible, hydrophilic PEG architecture supports solubility and reduces nonspecific interactions in bioconjugation and materials contexts, while the dual alkyne functionality enables crosslinking, multivalent labeling, and network formation when paired with azide-bearing partners. As a research-grade linker and crosslinker precursor, NH-bis(PEG2-propargyl) is commonly used to build modular PEGylated constructs for probe development, polymer conjugation, and surface functionalization strategies.

1. Multivalent Probe Labeling

NH-bis(PEG2-propargyl) is frequently used to introduce PEG-spaced alkyne handles for multivalent labeling of azide-functional biomolecules, affinity reagents, and imaging probes. In chemical biology workflows, the reagent’s two propargyl termini help increase effective local density of reactive sites, which is valuable when assembling higher-order probe architectures such as azide-tagged targeting ligands combined with alkyne-bearing reporter components. The PEG2 spacing is also leveraged to improve aqueous handling and to moderate steric effects during downstream conjugation steps, supporting robust probe assembly for biochemical assays and molecular imaging reagent development.

2. PEGylated Bioconjugation Linkers

NH-bis(PEG2-propargyl) serves as a practical PEGylated linker for constructing azide–alkyne conjugates used in labeling platforms for proteins, peptides, and nucleic-acid-associated reagents. Researchers commonly incorporate this bifunctional alkyne into workflows where a hydrophilic spacer is required to tune solubility, reduce aggregation, and maintain accessibility of functional groups on the final conjugate. Because the reagent is designed for click-compatible coupling, it is well suited to iterative build strategies in which azide-bearing biomolecular components are connected to alkyne-functional partners to generate defined PEGylated conjugation products for analytical chemistry and chemical biology tool development.

3. Hydrogel And Polymer Crosslinking

NH-bis(PEG2-propargyl) is used as a PEG-based crosslinker precursor in hydrogel and polymer network fabrication where azide-functional macromers or crosslinking partners are available. By providing two terminal alkyne groups, the reagent can participate in CuAAC-mediated network formation, enabling the creation of PEG-containing materials with tunable mechanical and swelling characteristics driven by the number and spacing of crosslinkable sites. In biomaterials research, this architecture is often selected to support water-rich environments and to facilitate subsequent functionalization of the material with azide-tagged biomolecules, allowing the development of modular scaffold systems for in vitro assay formats and advanced materials characterization.

4. Surface Functionalization For Assays

NH-bis(PEG2-propargyl) is applied to functionalize surfaces and solid supports that require stable, click-reactive alkyne presentation for subsequent azide-driven immobilization. In diagnostic reagent development and assay engineering, the reagent can be used to install PEG-spaced propargyl groups onto substrates such as polymer films, beads, or sensor surfaces, enabling controlled attachment of azide-bearing capture molecules, reporters, or affinity reagents through CuAAC. The hydrophilic PEG2 segment helps reduce unwanted surface adsorption and supports consistent conjugation behavior across heterogeneous solid phases, making NH-bis(PEG2-propargyl) a useful building block for assembling reusable assay components and research-grade diagnostic platforms.