N-(Aminooxy-PEG2)-N-bis(PEG3-propargyl) | CAS 2112737-71-8

N-(Aminooxy-PEG2)-N-bis(PEG3-propargyl) is a PEG-based, multifunctional click chemistry reagent designed for copper-free bioorthogonal conjugation workflows. It combines an aminooxy handle with two propargyl groups, enabling orthogonal chemical coupling strategies that are commonly used to functionalize biomolecules, surfaces, and imaging/diagnostic platforms. The flexible PEG architecture supports water compatibility and reduces nonspecific interactions, making the reagent attractive for constructing well-defined multivalent conjugates in chemical biology and molecular imaging research.

1. Biomolecule Conjugation

N-(Aminooxy-PEG2)-N-bis(PEG3-propargyl) is used to build multivalent conjugates for labeling proteins, peptides, and other biomolecular carriers in chemical biology workflows. Researchers leverage the dual propargyl functionality to introduce click-reactive sites that can be subsequently coupled to azide-bearing partners, supporting efficient assembly of probes, affinity reagents, or modular research tools. The aminooxy functionality provides an additional orthogonal coupling handle, which is particularly useful when sequential or site-specific attachment strategies are required for maintaining probe performance and preserving binding or recognition properties.

2. Molecular Imaging Probes

N-(Aminooxy-PEG2)-N-bis(PEG3-propargyl) is commonly incorporated into molecular imaging probe development where stable, well-controlled conjugation chemistry is needed to attach imaging moieties to targeting scaffolds or nanoparticle platforms. The PEG spacer and bifunctional propargyl groups support the creation of multivalent imaging constructs, which can improve labeling density and signal organization at the level of probe architecture. In imaging reagent pipelines, the aminooxy handle is often used to enable orthogonal attachment to carbonyl-containing components or pre-functionalized surfaces, helping teams streamline probe synthesis while maintaining consistent conjugate geometry.

3. Surface Functionalization and Coatings

N-(Aminooxy-PEG2)-N-bis(PEG3-propargyl) is applied to functionalize material surfaces and to generate click-ready coatings for biosensing and biomaterials research. The reagent’s PEG-rich structure helps promote aqueous compatibility and reduces detrimental surface interactions, which is valuable when immobilizing biomolecular recognition elements or assembling layered functional films. Dual propargyl groups allow subsequent attachment to azide-modified materials or capture chemistries, enabling reproducible fabrication of patterned or multicomponent surfaces for research-grade diagnostic reagent development.

4. Diagnostic Reagent Building Blocks

N-(Aminooxy-PEG2)-N-bis(PEG3-propargyl) serves as a versatile building block for constructing diagnostic and assay reagents that require modular conjugation of multiple components. Teams developing assay reagents use the reagent to introduce click-reactive handles that can be coupled to azide-functional labels, reporters, or solid-phase capture elements to achieve controlled reagent architecture. The aminooxy functionality supports orthogonal assembly schemes, which is especially helpful for creating standardized reagent formats where consistent labeling density and conjugate structure are important for downstream assay development and characterization workflows.