N-(Amino-PEG1)-N-bis(PEG2-propargyl) HCl salt | CAS 2100306-47-4

N-(Amino-PEG1)-N-bis(PEG2-propargyl) HCl salt is a PEGylated, amine-functionalized bis-propargyl click chemistry reagent designed for copper(I)-catalyzed azide–alkyne cycloaddition (CuAAC) workflows. The molecule couples a primary amine handle with two terminal propargyl groups separated by flexible PEG spacers, enabling efficient multivalent labeling and conjugation in aqueous research settings. Its PEG-rich architecture is commonly used to improve solubility, reduce nonspecific interactions, and support the construction of modular bioconjugates, imaging probes, and functional biomaterials.

1. Multivalent Probe Conjugation

N-(Amino-PEG1)-N-bis(PEG2-propargyl) HCl salt is widely used to build multivalent chemical probes by installing two alkyne termini onto azide-bearing targeting motifs, affinity ligands, or reporter constructs via CuAAC. Researchers in chemical biology and molecular imaging frequently select this reagent when they need higher local labeling density than mono-propargyl linkers, while the PEG spacers help maintain probe behavior in protein-rich buffers. The remaining amine functionality further supports downstream coupling to activated esters, isothiocyanates, or other amine-reactive chemistries, allowing probe sets where one handle is reserved for orthogonal assembly.

2. Surface Functionalization For Materials

N-(Amino-PEG1)-N-bis(PEG2-propargyl) HCl salt supports click-enabled modification of biomaterial and interface platforms, including PEG-tethered coatings, polymer surfaces, and hydrogel networks that incorporate azide groups. Materials scientists use the reagent to introduce dual alkyne sites that can be crosslinked, patterned, or integrated into multilayer constructs through CuAAC with azide-functional components. The PEG2-propargyl spacing is particularly relevant for creating stable, hydrated surfaces that can better control nonspecific adsorption and maintain accessibility of reactive groups during iterative material assembly.

3. Protein And Biomolecule Labeling

N-(Amino-PEG1)-N-bis(PEG2-propargyl) HCl salt is commonly employed for labeling biomolecules that have been engineered or derivatized with azide functionalities, enabling efficient attachment of PEGylated linkers and reporters for downstream detection and assay development. In bioconjugation workflows, the bis-propargyl design allows dual-point attachment strategies that can improve conjugate stability and reduce positional heterogeneity compared with single-site reagents. The amine handle offers an additional degree of freedom for conjugate design, such as incorporating the linker into amine-reactive capture schemes or preparing conjugates for orthogonal modifications.

4. Diagnostic Reagent Building Blocks

N-(Amino-PEG1)-N-bis(PEG2-propargyl) HCl salt is used as a modular building block for diagnostic and analytical reagent development where azide–alkyne click assembly is preferred for rapid, robust conjugation in aqueous systems. Diagnostic reagent developers incorporate this reagent to create multivalent labeling reagents, such as azide-functional capture elements paired with alkyne-containing reporter scaffolds, improving signal organization and reagent architecture. The PEG-rich linker design helps maintain reagent solubility and reduces aggregation tendencies during storage and handling, while the amine functionality supports coupling to additional chemistries used in assay formats and reagent cartridge workflows.