Hydroxy-Amino-bis(PEG2-propargyl) | CAS 2100306-77-0

Hydroxy-Amino-bis(PEG2-propargyl) is a multifunctional, PEG-based click chemistry reagent featuring two propargyl termini and an internal hydroxy–amino functionality. As a bis-propargyl compound, it is commonly used as a building block for copper-catalyzed azide–alkyne cycloaddition (CuAAC) and related alkyne-driven conjugation workflows. Its PEG2 spacing and hydrophilic character make it particularly relevant when researchers need to introduce two reactive alkyne handles for multivalent labeling, surface functionalization, or probe assembly in chemical biology and biomaterials programs.

1. Multivalent Probe Labeling

Hydroxy-Amino-bis(PEG2-propargyl) is widely used to generate multivalent chemical probes by coupling to azide-bearing targeting ligands, affinity tags, or imaging handles through CuAAC. The presence of two propargyl groups enables dense functionalization strategies that improve labeling efficiency in assay development and molecular imaging reagent preparation, while the PEG2 chain segments help maintain solubility and reduce nonspecific aggregation during probe handling. Researchers commonly incorporate this reagent into workflows for building modular probe libraries where azide partners can be varied while keeping a consistent bis-alkyne scaffold.

2. Biomolecule And Peptide Conjugation

Hydroxy-Amino-bis(PEG2-propargyl) supports click-based conjugation of biomolecules by providing two alkyne reactive sites that can be installed onto azide-functionalized biomolecular constructs. In chemical biology and proteomics tool development, the bis-propargyl format is useful for creating crosslinked or dual-labeled reagents, such as bifunctional tags for orthogonal downstream processing or for preparing standardized conjugates for assay panels. The PEG2 spacing and hydroxy–amino core are also advantageous in preparing aqueous conjugation mixtures where stable handling and reproducible coupling are important for research-grade reagent workflows.

3. Surface Functionalization For Biomaterials

Hydroxy-Amino-bis(PEG2-propargyl) is used to functionalize polymeric or hydrogel surfaces that carry azide groups, enabling the attachment of bioactive moieties, affinity ligands, or reporter groups via click chemistry. The dual alkyne functionality supports higher surface coverage and multivalent presentation of attached components, which is valuable in biomaterials science for building patterned or densely functionalized materials used in extracellular matrix mimics, cell-interaction studies, and material-based screening platforms. Its PEG2 character helps maintain surface wettability and facilitates consistent reagent performance during coating and washing steps typical of materials preparation.

4. Dual-Handle Linker Construction

Hydroxy-Amino-bis(PEG2-propargyl) serves as a practical dual-handle linker for constructing complex conjugates where two separate azide-bearing components must be incorporated into a single assembly. This usage is common in molecular imaging reagent development and diagnostic reagent chemistry, where researchers need controlled architectures such as bis-functional scaffolds for attaching two reporters, two affinity elements, or a reporter plus a capture handle. The reagent’s bis-propargyl design supports modular assembly strategies that streamline synthesis planning for research tool development, while the PEG2 spacing provides flexibility for reducing steric constraints in multicomponent coupling designs.