Fluorescein hydrazide | 109653-47-6

Fluorescein hydrazide, a versatile fluorescent compound utilized in diverse scientific applications, offers a multitude of possibilities.

Fluorescent Labeling: Widely recognized as a fluorescent tag for biomolecules like proteins, peptides, and nucleic acids, fluorescein hydrazide plays a pivotal role in visualization using fluorescence microscopy and flow cytometry. This labeling strategy allows researchers to explore cellular localization, protein interactions, and dynamic processes within living cells with unparalleled sensitivity and specificity.

Reactive Oxygen Species (ROS) Detection: Serving as a probe for detecting reactive oxygen species in biological samples, fluorescein hydrazide transforms upon interaction with ROS, producing a highly fluorescent product that facilitates quantification and imaging of oxidative stress in cells and tissues. This application is essential for deciphering the impact of oxidative stress on diseases such as cancer, neurodegeneration, and cardiovascular disorders.

Glycation Studies: In the realm of protein glycation research, fluorescein hydrazide plays a crucial role in monitoring protein-sugar interactions that lead to the formation of advanced glycation end-products (AGEs). Through labeling glycated proteins, researchers can meticulously track and quantify glycation events, shedding light on the pathogenesis of diabetic complications and age-related diseases.

Enzyme Assays: Employed in enzymatic assays to evaluate the activity of diverse enzymes, fluorescein hydrazide offers a fluorescence-based approach for precise monitoring of enzyme activities, including proteases, kinases, and oxidases. By detecting and quantifying fluorescein release upon enzyme action, researchers can gain insights into the intricate workings of these enzymatic processes with exceptional accuracy and reliability.