Cyanine5.5 hydrazide
Catalog Number | R05-0009 |
Category | Hydrazides |
Molecular Formula | C40H46Cl2N4O |
Molecular Weight | 669.73 |
Catalog Number | Size | Price | Quantity |
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R05-0009 | -- | $-- |
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Product Introduction
Cyanine5.5 hydrazide is a carbonyl-reactive near infrared dye. Hydrazides quickly react with carbonyl compounds, such as aldehydes and ketones, and form stable hydrazones. This reaction is useful for the preparation of labeled conjugates.Carbonyl groups in biomolecules can form spontaneously after oxidative stress or deamination of proteins. Alternatively, aldehyde groups can be specifically generated by periodate oxidation of sugar residues of glycoproteins, for example antibodies. Antibodies can be oxidized by sodium periodate and labeled with Cyanine5.5 hydrazide to form Cyanine5.5 labeled antibodies. Since sugar residues in antibodies are located far from variable, epitope-binding site, this method is particularly good for the labeling of antibodies.
Chemical Information |
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Related CAS | 2280971-80-2 (without anion) |
Purity | NMR 1H, HPLC-MS (95%) |
IUPAC Name | [6-[1,1-dimethyl-2-[5-(1,1,3-trimethylbenzo[e]indol-3-ium-2-yl)penta-2,4-dienylidene]benzo[e]indol-3-yl]hexanoylamino]azanium;dichloride |
Canonical SMILES | CC1(C(=[N+](C2=C1C3=CC=CC=C3C=C2)C)C=CC=CC=C4C(C5=C(N4CCCCCC(=O)N[NH3+])C=CC6=CC=CC=C65)(C)C)C.[Cl-].[Cl-] |
InChI | InChI=1S/C40H44N4O.2ClH/c1-39(2)34(43(5)32-25-23-28-16-11-13-18-30(28)37(32)39)20-8-6-9-21-35-40(3,4)38-31-19-14-12-17-29(31)24-26-33(38)44(35)27-15-7-10-22-36(45)42-41;;/h6,8-9,11-14,16-21,23-26H,7,10,15,22,27,41H2,1-5H3;2*1H |
InChI Key | OZQXTPNFHISECI-UHFFFAOYSA-N |
Solubility | practically insoluble in water (< 2 uM), good in polar organic solvents (DMF, DMSO, alcohols) |
Appearance | dark blue powder |
- Product Specification
- Application
ε, L⋅mol-1⋅cm-1 | 198000 |
Fluorescence Quantum Yield | 0.2 |
Excitation | 684 |
Emission | 710 |
Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Cyanine55 hydrazide, a fluorescent dye utilized in various bioscience applications, particularly excels in imaging and labeling. Here are four key applications of Cyanine55 hydrazide:
Molecular Imaging: Delving into the realm of molecular imaging, Cyanine55 hydrazide emerges as a pivotal tool for visualizing real-time biological processes. This versatile dye can be seamlessly conjugated to biomolecules like antibodies or peptides, enabling researchers to meticulously trace their distribution and interactions within live cells or tissues. Such precision is paramount for unraveling the intricacies of disease progression, optimizing drug delivery, and deciphering the dynamic landscape of cellular activities.
Fluorescence Microscopy: In the domain of fluorescence microscopy, Cyanine55 hydrazide shines as a beacon for labeling and pinpointing specific targets within a sample. Its luminous fluorescence and steadfast stability render it a top choice for achieving high-resolution imaging of cellular structures, proteins, and nucleic acids. This technique serves as a cornerstone for scrutinizing cellular architecture, unraveling protein localization, and elucidating gene expression patterns in a visually captivating manner.
Bioconjugation: Unlocking a realm of possibilities, Cyanine55 hydrazide can be seamlessly conjugated to a myriad of biomolecules – be it proteins, nucleic acids, or small molecules – to craft fluorescent probes. These probes serve as invaluable instruments for detecting and quantifying specific targets in biochemical assays like ELISA and flow cytometry, ensuring meticulous identification and quantification of biomolecules amidst complex mixtures, paving the way for groundbreaking discoveries.
Optical Imaging in Vivo: Transitioning to the realm of animal studies, Cyanine55 hydrazide takes center stage for optical imaging applications, allowing researchers to monitor biological processes within living organisms with unparalleled clarity. Its near-infrared fluorescence capability grants deep tissue penetration and minimal background interference, making it a prime candidate for non-invasive imaging endeavors. Through this lens, researchers gain insights into tumor progression, metastasis dynamics, and the efficacy of therapeutic interventions with unprecedented precision and depth.
Computed Properties | |
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Hydrogen Bond Donor Count | 2 |
Hydrogen Bond Acceptor Count | 4 |
Rotatable Bond Count | 9 |
Exact Mass | 668.3048675 g/mol |
Monoisotopic Mass | 668.3048675 g/mol |
Topological Polar Surface Area | 63Ų |
Heavy Atom Count | 47 |
Formal Charge | 0 |
Complexity | 1190 |
Isotope Atom Count | 0 |
Defined Atom Stereocenter Count | 0 |
Undefined Atom Stereocenter Count | 0 |
Defined Bond Stereocenter Count | 0 |
Undefined Bond Stereocenter Count | 3 |
Covalently-Bonded Unit Count | 3 |
Compound Is Canonicalized | Yes |
Applications of Fluorescent Probes & Dyes
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