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Cyanine3 NHS ester
| Catalog Number | R01-0016 |
| Category | NHS Esters |
| Molecular Formula | C34H40N3BF4O4 |
| Molecular Weight | 641.5 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.Cyanine3 NHS ester is a replacement for NHS esters of Cy3®, Alexa Fluor 546, and DyLight 549.
Chemical Information
Product Specification
Computed Properties
| Related CAS | 1393427-85-4 (without anion) |
| Synonyms | Cy3 NHS ester; 2-((E)-3-((E)-1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethylindolin-2-ylidene)prop-1-en-1-yl)-1,3,3-trimethyl-3H-indol-1-ium chloride |
| Purity | NMR 1H and HPLC-MS (95+%) |
| IUPAC Name | (2,5-dioxopyrrolidin-1-yl) 6-[(2E)-3,3-dimethyl-2-[(E)-3-(1,3,3-trimethylindol-1-ium-2-yl)prop-2-enylidene]indol-1-yl]hexanoate;chloride |
| Canonical SMILES | CC1(C2=CC=CC=C2[N+](=C1C=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)ON5C(=O)CCC5=O)(C)C)C)C.[Cl-] |
| InChI | InChI=1S/C34H40N3O4.ClH/c1-33(2)24-14-8-10-16-26(24)35(5)28(33)18-13-19-29-34(3,4)25-15-9-11-17-27(25)36(29)23-12-6-7-20-32(40)41-37-30(38)21-22-31(37)39;/h8-11,13-19H,6-7,12,20-23H2,1-5H3;1H/q+1;/p-1 |
| InChIKey | KWDMHANYPGHTLW-UHFFFAOYSA-M |
| Solubility | poorly soluble in water (2.3 mM = 1.5 g/L), soluble in organic solvents (DMF, DMSO, dichloromethane) |
| Appearance | red powder |
| ε, L⋅mol-1⋅cm-1 | 150000 |
| Fluorescence Quantum Yield | 0.31 |
| Excitation | 555 |
| Emission | 570 |
| Hydrogen Bond Donor Count | 0 |
| Hydrogen Bond Acceptor Count | 6 |
| Rotatable Bond Count | 10 |
| Exact Mass | 589.2707345 g/mol |
| Monoisotopic Mass | 589.2707345 g/mol |
| Topological Polar Surface Area | 69.9Ų |
| Heavy Atom Count | 42 |
| Formal Charge | 0 |
| Complexity | 1120 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 2 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 2 |
| Compound Is Canonicalized | Yes |
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