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BDP 558/568 alkyne
| Catalog Number | R02-0013 |
| Category | Alkynes |
| Molecular Formula | C19H16N3BF2OS |
| Molecular Weight | 383.22 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| R02-0013 | -- | $-- |
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Product Introduction
BDP 558/568 is a borondipyrromethene dye for Cyanine3 channel. The dye possesses high quantum yield. This derivative is a terminal alkyne for Copper-catalyzed Click chemistry.
Chemical Information |
|
|---|---|
| Purity | NMR 1H, HPLC-MS (95%) |
| IUPAC Name | 3-(2,2-difluoro-12-thiophen-2-yl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaen-4-yl)-N-prop-2-ynylpropanamide |
| Canonical SMILES | [B-]1(N2C(=CC=C2CCC(=O)NCC#C)C=C3[N+]1=C(C=C3)C4=CC=CS4)(F)F |
| InChI | InChI=1S/C19H16BF2N3OS/c1-2-11-23-19(26)10-8-14-5-6-15-13-16-7-9-17(18-4-3-12-27-18)25(16)20(21,22)24(14)15/h1,3-7,9,12-13H,8,10-11H2,(H,23,26) |
| InChI Key | PQDOJARDWWICEJ-UHFFFAOYSA-N |
| Solubility | well soluble in DCM, DMF, DMSO, acetone, low aqueous solubility |
| Appearance | dark colored solid |
- Product Specification
- Application
| ε, L⋅mol-1⋅cm-1 | 84400 |
| Excitation | 561 |
| Emission | 569 |
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
BDP 558/568 alkyne, a versatile fluorescent dye, finds widespread usage in diverse bioimaging and labeling scenarios. Here are four key applications of BDP 558/568 alkyne:
Fluorescence Microscopy: Employing BDP 558/568 alkyne in fluorescence microscopy enhances the visualization of cellular components and structures. By selectively tagging biomolecules, researchers can monitor dynamic cellular processes in real-time. The exceptional brightness and enduring photostability of this dye render it an optimal choice for prolonged imaging endeavors.
Flow Cytometry: Within the realm of flow cytometry, BDP 558/568 alkyne emerges as a crucial tool for cell labeling, enabling comprehensive multiparametric analysis. This labeling strategy empowers scientists to evaluate diverse cellular traits like size, granularity, and specific surface markers. Moreover, its seamless integration with other fluorescent tags facilitates the creation of intricate staining arrays to investigate varied cell populations.
Protein Labeling: In the domain of biochemical assays, BDP 558/568 alkyne plays a pivotal role in site-specific protein labeling, fostering precise insights into protein dynamics. Leveraging click chemistry, researchers can conjugate the dye to proteins with unparalleled accuracy, preserving protein functionality intact. This methodology proves indispensable for scrutinizing protein interactions, behaviors, and subcellular localizations, offering profound insights into cellular machinations.
Live-Cell Imaging: By enabling live-cell labeling without compromising cellular viability, BDP 558/568 alkyne fuels cutting-edge studies in live-cell imaging. Researchers can observe and analyze pivotal cellular processes such as migration, division, and signal transduction within live cells. This application stands as a cornerstone for deciphering the intricacies of cellular behavior within a physiological context, shedding light on fundamental biological phenomena.
Computed Properties | |
|---|---|
| Hydrogen Bond Donor Count | 1 |
| Hydrogen Bond Acceptor Count | 5 |
| Rotatable Bond Count | 5 |
| Exact Mass | 383.1075198 g/mol |
| Monoisotopic Mass | 383.1075198 g/mol |
| Topological Polar Surface Area | 65.3Ų |
| Heavy Atom Count | 27 |
| Formal Charge | 0 |
| Complexity | 785 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
Applications of Fluorescent Probes & Dyes
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