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ATTO 647N DMPE
| Catalog Number | F10-0091 |
| Category | ATTO |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| F10-0091 | -- | $-- |
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Product Introduction
ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, excellent fluorescence quantum yield, high photostability, excellent ozone resistance, good solubility, and very little triplet formation. ATTO 647N is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. In common with most ATTO-labels, absorption and fluorescence are independent of pH in the range of 2 to 11, used in typical applications. As supplied ATTO 647N consists of a mixture of two isomers with practically identical absorption and fluorescence properties.ATTO-Dye Labeled Phospholipids Sigma-Aldrich offers a variety of glycero-phospholipids carrying one or two fatty acid groups (lipophilic groups) and a phosphate ester residue (hydrophilic group). They are labeled at the hydrophilic head group. After incorporation of the phospholipid into a membrane the fluorophore is located at the water/lipid interface of the membrane. We currently provide 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), palmitoyl-sn-glycero-phosphoethanolamine (PPE), and 1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine (DMPE) labeled with ATTO-dyes.find more information here
Chemical Information |
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|---|---|
| Purity | ≥90.0% (HPCE) |
| NACRES | NA.32 |
- Product Specification
- Application
| Excitation | 643 |
| Emission | 665 ±5 |
| Properties Quality Level | 100 |
| Storage | −20 °C |
ATTO 647N DMPE, a fluorescent dye-labeled phospholipid, widely embraced in diverse bioscience realms. Explore the following key applications of ATTO 647N DMPE:
Fluorescence Microscopy: Seize the power of ATTO 647N DMPE in fluorescence microscopy, a pivotal tool for visualizing cell membranes and lipid dynamics. By embedding this vivid dye into lipid membranes, researchers embark on a journey to decipher membrane organization and mobility within live cells. This breakthrough sheds light on cellular phenomena like endocytosis, exocytosis, and cell signaling.
Membrane Studies: Delve into the intricate world of lipid bilayers and membrane architecture, with ATTO 647N DMPE as your guide. This luminary phospholipid unveils a treasure trove of insights into membrane fluidity and phase behavior. Scientists harness this labeled phospholipid to explore the impact of diverse lipids, proteins, and environmental factors on membrane characteristics, enriching our comprehension of membrane biophysics and the pivotal role of lipids in cellular orchestration.
Super-Resolution Imaging: Behold the transformative capabilities of ATTO 647N DMPE in the realm of super-resolution microscopy techniques like STED, PALM, and STORM. Armed with this dye, researchers unveil high-definition images of subcellular structures and lipid domains that elude traditional microscopy boundaries. This breakthrough avenue opens up vistas for in-depth investigations into molecular interactions and cellular intricacies at the nano level, presenting a new lens to fathom the intricate architecture of life.
Applications of Fluorescent Probes & Dyes
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