ATTO 425-Biotin

ATTO 425-Biotin is a fluorescent dye that is conjugated with biotin, offering a range of applications in the bioscience field. Here are some key applications of ATTO 425-Biotin:

Fluorescence Microscopy: ATTO 425-Biotin is widely used in fluorescence microscopy for labeling biotinylated molecules. When coupled with streptavidin or avidin conjugates, it provides highly specific and bright labeling of target proteins, nucleic acids, or other biotinylated entities. This allows researchers to visualize cellular structures and molecular interactions with high resolution and sensitivity.

Flow Cytometry: In flow cytometry, ATTO 425-Biotin is utilized for the detection and quantification of biotinylated cell surface markers. By tagging these markers with fluorescent-labeled streptavidin, researchers can analyze cell populations based on the presence and amount of specific antigens. This application is pivotal for immunophenotyping, cell sorting, and functional studies of cell populations.

Protein-Protein Interactions: ATTO 425-Biotin can be used in assays to study protein-protein interactions through biotin-streptavidin binding. By biotinylating one of the interacting proteins and using streptavidin conjugated with ATTO 425, researchers can detect and quantify the interaction using fluorescence techniques. This method is particularly useful in validating binding events and in the high-throughput screening of interaction inhibitors.

Immunoassays: In various immunoassay formats, ATTO 425-Biotin serves as a versatile and highly sensitive detection reagent. It can be conjugated to antibodies or antigens, allowing for fluorescent signal generation upon binding. This application enhances the sensitivity and quantification capabilities of assays such as ELISA, western blotting, and lateral flow tests in diagnostics and research.