5-TAMRA NHS Ester

Name: 5-TAMRA NHS Ester
Brand: BOC Sciences
Rating: 5 (1 reviews)

Catalog Number	F07-0003
Category	TAMRA Dyes
Molecular Formula	C29H25N3O7
Molecular Weight	527.5

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Product Introduction

Although the mixed isomers of 5(6)-TAMRA Acid is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA is more often used for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids. 5-TAMRA dye is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.
The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Chemical Information
Product Specification
Application

Chemical Information

Solubility

DMSO, DMF, MeOH

Product Specification

Excitation	553
Emission	575
Storage	-20 °C

Application

5-TAMRA NHS Ester is a reactive TAMRA (tetramethylrhodamine) succinimidyl ester designed for covalent fluorescent labeling of primary amines under standard bioconjugation conditions. Its NHS ester reacts efficiently with lysine residues and N-termini in proteins and peptides, enabling bright red/orange fluorescence for imaging, labeling, and assay development. Because the dye is introduced as an activated ester, researchers commonly use it to prepare fluorescent conjugates for microscopy, flow cytometry, and fluorescence-based readouts.

1. Protein And Peptide Labeling

5-TAMRA NHS Ester is widely used to fluorescently label antibodies, enzymes, and other amine-containing proteins for downstream binding studies and fluorescence imaging workflows. By targeting lysine side chains and N-termini, the reagent supports preparation of labeled bioconjugates used in immunostaining, ligand-binding assays, and protein interaction experiments where a red-emitting fluorophore is advantageous for multiplexing or background separation.

2. Fluorescence Microscopy Staining

5-TAMRA NHS Ester is commonly incorporated into fluorescent labeling protocols for cellular imaging experiments that require a TAMRA-class dye. Researchers use the NHS ester to generate amine-reactive staining conjugates for labeling biomolecules associated with cells and extracellular targets, supporting fluorescence microscopy readouts in fixed-sample studies and imaging workflows where covalent attachment improves retention during washing steps.

3. Flow Cytometry Conjugates

5-TAMRA NHS Ester is used to prepare fluorescent antibody or protein conjugates for flow cytometry panels that rely on red/orange emission. In these workflows, the NHS-ester chemistry enables efficient attachment to amine-bearing targeting reagents, allowing researchers to track binding to cell-associated epitopes and to build multicolor assays where TAMRA emission helps distribute fluorophores across detection channels.

4. Fluorescent Bioassay Reagents

5-TAMRA NHS Ester supports the development of fluorescence-based assay reagents by providing a convenient route to label assay components such as enzymes, reporters, or binding proteins with a stable covalent fluorophore. Teams developing fluorescence assays and binding readouts often incorporate TAMRA-labeled conjugates to track reagent distribution, monitor binding events, or generate fluorescent standards for assay calibration and instrument verification.

5. Surface and Biomaterial Functionalization

5-TAMRA NHS Ester is also used in biomaterials and surface modification workflows where amine-containing materials or coatings are available. Researchers apply the NHS ester to functionalize polymers, beads, or other amine-terminated substrates with a fluorescent tag, enabling visualization of surface-associated components and supporting materials characterization by fluorescence microscopy or bulk fluorescence measurements.