4,7,10,13,16,19,22,25,28,31,34,37-Dodecaoxatetraconta-1,39-diyne | CAS 1351373-49-3

4,7,10,13,16,19,22,25,28,31,34,37-Dodecaoxatetraconta-1,39-diyne is a long-chain, poly(ethylene glycol)-like diyne click chemistry reagent featuring two terminal alkyne handles separated by an extended ether-rich scaffold. As a diyne building block suited for alkyne-based click conjugation workflows, it is commonly selected to introduce rigid, distance-controlled linkers into probes, imaging agents, and functional biomaterials. The flexible oxygen-rich backbone helps solubilize and reduce nonspecific interactions while the two alkynes enable multivalent or modular coupling strategies in chemical biology and materials research.

1. Multivalent Probe Conjugation

4,7,10,13,16,19,22,25,28,31,34,37-Dodecaoxatetraconta-1,39-diyne is frequently used as a linker to build multivalent chemical probes where two alkyne functionalities are required for sequential or orthogonal conjugation steps. Research groups in chemical biology and molecular imaging rely on diyne reagents to tune effective valency and spatial presentation of targeting motifs, fluorophores, affinity ligands, or capture groups on a single construct. The ether-rich backbone supports aqueous compatibility, which is advantageous when assembling probe libraries for screening or comparative studies of labeling density and linker length effects.

2. Surface Functionalization Linkers

4,7,10,13,16,19,22,25,28,31,34,37-Dodecaoxatetraconta-1,39-diyne is well suited for functionalizing solid supports and biomaterial surfaces that require controlled attachment of alkyne-bearing moieties. Materials scientists use diyne linkers to create patterned or multivalent coatings on polymer films, hydrogel scaffolds, and sensor interfaces, enabling downstream immobilization of reactive partners through click-compatible coupling chemistry. The extended, oxygen-rich spacer helps maintain accessibility of surface-bound ligands and can improve colloidal stability during surface modification workflows, which is important for reproducible fabrication of diagnostic reagents and research-grade materials.

3. Biomolecular Labeling Platforms

4,7,10,13,16,19,22,25,28,31,34,37-Dodecaoxatetraconta-1,39-diyne is commonly incorporated into labeling platforms that require a defined, flexible distance between biomolecular recognition elements and the final reporter group. In biomaterials science and chemical biology, the reagent’s two terminal alkyne handles enable modular assembly of conjugates where one end can be reserved for further functionalization while the other end is used to attach to a biomolecule-derived scaffold, such as polymeric carriers or affinity-tagged constructs. This approach supports consistent construct architecture across experiments, helping researchers compare conjugate performance in assays that depend on linker length and presentation geometry.

4. Molecular Imaging Reagent Building

4,7,10,13,16,19,22,25,28,31,34,37-Dodecaoxatetraconta-1,39-diyne is used as a practical building block for constructing imaging reagent candidates that benefit from multivalent or distance-tuned labeling. Molecular imaging and probe development workflows often require linkers that can be appended to fluorophores, quencher systems, or other reporter modules while maintaining solubility and minimizing aggregation. The long, ether-rich diyne scaffold supports robust conjugation design, enabling researchers to generate structurally consistent probe formats for optimization of signal readouts and for comparative studies across labeling densities and linker configurations.

5. Diagnostic Assay Reagent Assembly

4,7,10,13,16,19,22,25,28,31,34,37-Dodecaoxatetraconta-1,39-diyne is adopted in the assembly of diagnostic assay reagents where click-compatible modularity is valuable for constructing capture-and-detection architectures. Diagnostic reagent developers use diyne linkers to connect assay components that carry complementary reactive partners, building multivalent conjugates that can improve reagent handling and reproducibility during assay formulation. The extended polyether-like spacer helps maintain accessibility of reactive sites and can support stable reagent preparation in aqueous assay buffers, which is important for consistent performance in laboratory testing workflows.