3'-(6-TAMRA) CPG 1000

3'-(6-TAMRA) CPG 1000 is a solid-phase, phosphoramidite-derived TAMRA labeling support designed for oligonucleotide synthesis and post-synthetic fluorescent tagging. This CPG-bound dye format enables straightforward incorporation of a TAMRA fluorophore at the 3′ terminus of DNA or RNA constructs, supporting fluorescence readouts in nucleic acid workflows where end-labeling position matters for hybridization, strand extension, or reporter placement. Researchers commonly use this reagent to generate fluorescently labeled probes and standards for microscopy, fluorescence-based nucleic acid assays, and imaging-compatible oligonucleotide reagents.

1. 3′-End Fluorescent Oligonucleotides

3'-(6-TAMRA) CPG 1000 supports the preparation of 3′-end TAMRA-labeled oligonucleotides used as fluorescent reporters in nucleic acid assays and molecular imaging workflows. Because the dye is presented on a solid support during synthesis, it is well aligned with routine oligonucleotide assembly practices used in academic and industrial laboratories. End-position labeling is particularly valuable when probe performance depends on maintaining binding geometry during hybridization or when the 3′ terminus must carry the fluorescent readout for downstream analysis.

2. Fluorescent Hybridization Probes

3'-(6-TAMRA) CPG 1000 is used to build TAMRA-labeled DNA probes for fluorescence detection of complementary nucleic acid targets. In hybridization-based assays, 3′-end labeling provides a convenient way to track probe presence and binding via fluorescence without requiring additional post-labeling steps. This format is frequently selected for developing probe sets for research workflows such as nucleic acid binding studies, assay development for fluorescence readouts, and fluorescence microscopy-compatible labeling of nucleic acid components.

3. Fluorescent Standards And Controls

3'-(6-TAMRA) CPG 1000 enables preparation of TAMRA-labeled oligonucleotide standards and assay controls used to validate labeling consistency and instrument-based fluorescence measurements. Laboratories often rely on defined fluorescent oligonucleotides to normalize signal across experiments, calibrate imaging or plate-reader workflows, and benchmark assay conditions when comparing fluorescence outputs from different probe batches. The solid-phase dye incorporation approach supports reproducible construction of end-labeled nucleic acid reagents used in routine fluorescence measurement pipelines.

4. Nucleic Acid Imaging Reagents

3'-(6-TAMRA) CPG 1000 is applied to generate fluorescently labeled oligonucleotides for cellular and molecular imaging workflows where TAMRA emission provides a convenient visible-channel reporter. Researchers use 3′-end TAMRA labeling to visualize nucleic acid localization or to tag nucleic acid components in imaging experiments that require a defined fluorophore position on the strand. This reagent is therefore used in fluorescence microscopy and related imaging development efforts that benefit from stable, covalently attached end labeling of oligonucleotide probes.