3,6,9,12,15-Pentaoxaoctadec-17-yn-1-ol mesylate | CAS 1036204-62-2

3,6,9,12,15-Pentaoxaoctadec-17-yn-1-ol mesylate is a propargyl-terminated, poly(ethylene glycol)-like click chemistry reagent designed for copper-catalyzed azide–alkyne cycloaddition (CuAAC) workflows. The mesylate leaving group enables efficient attachment to nucleophilic handles on biomolecules and surfaces, while the terminal alkyne provides a robust handle for subsequent conjugation to azide-bearing partners. Its long, ether-rich spacer improves solubility and reduces steric constraints, making it a common choice for building modular linkers in chemical biology, imaging, and materials labeling.

1. Surface And Bead Functionalization

3,6,9,12,15-Pentaoxaoctadec-17-yn-1-ol mesylate is widely used to introduce a CuAAC-reactive alkyne onto solid supports, including polymer beads and functionalized surfaces, where downstream azide coupling enables flexible assembly of probes and affinity reagents. The ether-rich chain helps maintain accessibility of the terminal alkyne after immobilization, which is particularly valuable for high-density labeling formats such as bead-based assays and flow-compatible materials. Researchers also employ this reagent to create reusable intermediate surfaces that can be rapidly remodeled by reacting with a library of azide-tagged ligands, enabling streamlined screening and method development in chemical biology and diagnostic reagent development.

2. Biomolecule Linker Construction

3,6,9,12,15-Pentaoxaoctadec-17-yn-1-ol mesylate supports the preparation of alkyne-functional biomolecule conjugates by providing a mesylate-activated handle for attachment to nucleophilic groups under standard bioconjugation conditions. Once installed, the terminal alkyne serves as a stable click handle for coupling to azide-bearing targets such as affinity tags, capture moieties, or imaging reporters. The long, hydrophilic spacer is frequently selected to improve conjugate behavior in aqueous buffers, helping reduce aggregation and steric interference that can otherwise limit labeling efficiency and preserve binding-site accessibility in complex bioconjugate architectures.

3. Molecular Imaging Probe Assembly

3,6,9,12,15-Pentaoxaoctadec-17-yn-1-ol mesylate is commonly incorporated as a modular linker in the synthesis of imaging and detection reagents that rely on CuAAC to connect an alkyne-bearing scaffold to azide-functional reporter components. The reagent’s flexible polyether spacer supports reliable presentation of the click handle, which is advantageous when assembling multi-component probe systems such as fluorescent conjugates, affinity-enriched imaging constructs, and platform reagents used in molecular imaging research. By enabling intermediate alkyne installation followed by azide coupling, it fits practical labeling workflows where probe components are exchanged or optimized across experiments without redesigning the entire conjugation strategy.

4. Diagnostic Reagent And Assay Platforms

3,6,9,12,15-Pentaoxaoctadec-17-yn-1-ol mesylate is used in the development of diagnostic reagent components and assay platform materials that require controlled, modular conjugation chemistry. The reagent can be used to create alkyne-functional intermediates that are later coupled to azide-tagged detection elements, such as reporter conjugates or capture reagents, using CuAAC as a reliable assembly step. Its hydrophilic spacer architecture is particularly useful for maintaining reagent solubility and minimizing nonspecific interactions in assay buffers, supporting consistent performance in multiplexed or sequential labeling schemes used during method development and reagent optimization in laboratory diagnostics and research assay development.