Dihydrorhodamine 123 | 109244-58-8

Catelog Number	A03-0012
Category	Nitric Oxide (NO) & Reactive Oxygen Species (ROS)
Molecular Formula	C21H18N2O3
Molecular Weight	346.4

Catalog Number	Size	Price	Quantity
A03-0012	--	$--

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Product Introduction

DHR is a cell-permeable fluorogenic probe that is used for detection of reactive oxygen species (ROS) such as intracellular peroxide and peroxynitrite formation. It is oxidized by peroxynitrite to produce the highly fluorescent compound rhodamine in vitro.

Chemical Information
Synonyms	DHR 123; 2-(3,6-diamino-9H-xanthen-9-yl)-benzoic acid methyl ester
Purity	≥98%
IUPAC Name	methyl 2-(3,6-diamino-9H-xanthen-9-yl)benzoate
Canonical SMILES	COC(=O)C1=CC=CC=C1C2C3=C(C=C(C=C3)N)OC4=C2C=CC(=C4)N
InChI	InChI=1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3
InChI Key	FNEZBBILNYNQGC-UHFFFAOYSA-N
Solubility	Soluble in DMSO
Appearance	Solid Powder

Product Specification
Excitation	500 nm
Emission	536 nm
Storage	Store at -20°C
Signal	Warning
GHS Hazard Statements	H302 (100%): Harmful if swallowed [Warning Acute toxicity, oral] H315 (100%): Causes skin irritation [Warning Skin corrosion/irritation] H319 (100%): Causes serious eye irritation [Warning Serious eye damage/eye irritation] H335 (100%): May cause respiratory irritation [Warning Specific target organ toxicity, single exposure; Respiratory tract irritation]
Precautionary Statement Codes	P261, P264, P264+P265, P270, P271, P280, P301+P317, P302+P352, P304+P340, P305+P351+P338, P319, P321, P330, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (The corresponding statement to each P-code can be found at the GHS Classification page.)

Computed Properties
XLogP3	3.4
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Exact Mass	346.13174244 g/mol
Monoisotopic Mass	346.13174244 g/mol
Topological Polar Surface Area	87.6Å²
Heavy Atom Count	26
Formal Charge	0
Complexity	488
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently-Bonded Unit Count	1
Compound Is Canonicalized	Yes

Literatures

PMID	Publication Date	Title	Journal
22248218	2012-09-01	Effect of priming in subpopulations of peripheral neutrophils from patients with chronic periodontitis	Journal of periodontology
22768920	2012-07-06	Effects of lipid-probe interactions in biochemical fluorometric methods that assess HDL redox activity	Lipids in health and disease
22497768	2012-07-01	A fluorimetric assay for human reticulocyte 15-lipoxygenase-1 activity	Analytical biochemistry
22545706	2012-06-01	DHR123: an alternative probe for assessment of ROS in human spermatozoa	Systems biology in reproductive medicine
22262438	2012-01-01	Measurement of oxidative burst in neutrophils	Methods in molecular biology (Clifton, N.J.)

Patents

Publication Number	Title	Priority Date
CN-114213419-A	Fluorescent probe with both fluorescence imaging and type I photodynamic/photothermal killing cancer cell activity, preparation method and application thereof	2021-11-29
CN-114213419-B	A fluorescent probe with both fluorescence imaging and type I photodynamic/photothermal killing activity on cancer cells and its preparation method and application	2021-11-29
WO-2022078524-A2	Specific conjugation of an antibody	2021-11-03
WO-2022078524-A4	Specific conjugation of an antibody	2021-11-03
CN-114129725-A	A kind of photodynamically triggered nitric oxide to release black phosphorus nanomaterial and its preparation method and application	2021-10-28