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HYDROETHIDINE | CAS 38483-26-0
| Catalog Number | A03-0011 |
| Category | NO & ROS Probes |
| Molecular Formula | C21H21N3 |
| Molecular Weight | 315.41 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes.
Chemical Information
Product Specification
Application
| Synonyms | Dihydroethidium (Hydroethidium);DHE; DIHYDROETHIDIUM; HYDROETHIDINE; HYDROETHIDINE(TM); 2,7-DIAMINO-10-ETHYL-9-PHENYL-9,10-DIHYDROPHENANTHRIDINE; 3,8-DIAMINO-5,6-DIHYDRO-5-ETHYL-6-PHENYLPHENANTHRIDINE; 5,6-dihydro-3,8-diamino-5-ethyl-6-phenyl-phenanthridin; 5,6-dihydro-3,8-diamino-5-ethyl-6-p |
| Purity | ≥95% |
| IUPAC Name | 5-ethyl-6-phenyl-6H-phenanthridine-3,8-diamine |
| Canonical SMILES | CCN1C(C2=C(C=CC(=C2)N)C3=C1C=C(C=C3)N)C4=CC=CC=C4 |
| InChI | InChI=1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3 |
| InChIKey | XYJODUBPWNZLML-UHFFFAOYSA-N |
| Solubility | Soluble in DMF or DMSO |
| Appearance | Pink solid |
| Excitation | 518 |
| Emission | 605 |
| Storage | Store at-10 to-35 °C, Desiccate |
Hydroethidine, a fluorescent dye renowned for its ability to detect superoxide production in biological systems, boasts a range of vital applications. Here are four key applications of Hydroethidine intricately:
Cellular Oxidative Stress Measurement: Prevailing as a cornerstone in cellular research, Hydroethidine serves as a potent tool for evaluating oxidative stress levels within cells by detecting intracellular superoxide. The dye seamlessly permeates cells and undergoes oxidation catalyzed by superoxide, culminating in the production of fluorescent ethidium. This fluorescence, quantifiable through flow cytometry or fluorescence microscopy, unveils valuable insights into cellular oxidative damage and the intricate interplay of antioxidant defenses.
Neuroscience Research: Within the realm of neuroscience, Hydroethidine emerges as a pivotal component in unraveling the enigma of oxidative stress in neurodegenerative ailments. By gauging superoxide levels in neuronal cultures or brain tissue, researchers embark on a journey to unravel the intricate ties between oxidative stress and afflictions like Parkinson’s disease and Alzheimer’s disease. This exploration aids in shedding light on disease pathology and assessing the efficacy of potential neuroprotective interventions.
Cardiovascular Studies: Positioned at the forefront of cardiovascular investigations, Hydroethidine plays a crucial role in assessing oxidative stress within heart tissues and blood vessels. This application facilitates in-depth explorations into the repercussions of oxidative stress on cardiovascular well-being, including its involvement in conditions such as hypertension, atherosclerosis, and heart failure. The resulting insights are essential for sculpting antioxidant-driven therapies and formulating strategic preventive measures aimed at safeguarding cardiovascular health.
Inflammation Research: Fostering breakthroughs in the domain of inflammation-related studies, Hydroethidine emerges as a key player in unraveling the intricate relationship between oxidative stress and inflammation across diverse disease models. By pinpointing superoxide presence within inflammatory cells, researchers gain profound insights into how oxidative stress fuels inflammatory responses and triggers tissue damage. This pivotal information aids in the discovery of innovative anti-inflammatory modalities and enhances our comprehension of the underlying mechanisms at play in inflammatory diseases.
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