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DAF-2 diacetate | CAS 205391-02-2
| Catalog Number | A21-0002 |
| Category | Nitric Oxide Probes |
| Molecular Formula | C24H18N2O7 |
| Molecular Weight | 446.4 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
DAF-2 diacetate is a cell-permeable nitric oxide indicator that emits green fluorescence upon reaction with NO. Suitable for live-cell imaging and physiological monitoring.
Chemical Information
Product Specification
Application
| Synonyms | DAF-2 DA; 4,5-Diaminofluorescein diacetate; 2-(3,6-diacetyloxy-4,5-diamino-9H-xanthen-9-yl)-benzoic acid |
| Purity | ≥95% |
| Canonical SMILES | CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=CC(=C(C=C5C(=O)O3)N)N |
| InChI | InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3 |
| InChIKey | PTSUYDXEEKDBQU-UHFFFAOYSA-N |
| Solubility | DMSO: <2 mg/mL，H2O: <2 mg/mL |
| Appearance | Colorless to white lyophilized powder |
| MDL Number | MFCD00063449 |
| NACRES | NA.47 |
| Excitation | 485 nm |
| Emission | 538 nm |
| Properties Quality Level | 100 |
| Storage | Store at -20°C |
DAF-2 diacetate, a fluorescent dye widely utilized in biological research, is instrumental in measuring nitric oxide (NO) production in cells and tissues. Here are four key applications of DAF-2 diacetate:
Cellular NO Detection: A cornerstone in cellular research, DAF-2 diacetate serves as a potent tool for detecting nitric oxide production within cells. Upon diffusion into cells, the dye undergoes deacetylation, transforming into a fluorescent entity that interacts with NO, enabling real-time visualization. This application sheds light on NO’s intricate involvement in cellular signaling and physiology, unraveling crucial insights into cellular activities.
Tissue Imaging: In the realm of tissue exploration, DAF-2 diacetate plays a pivotal role in studying the distribution of nitric oxide within tissues, yielding fluorescent outputs. This application proves particularly invaluable in vascular biology research, where NO exerts significant regulatory influence. Researchers leverage this dye to map NO production across diverse tissue types, offering profound insights into physiological and pathological conditions, enriching our understanding of tissue dynamics.
Pharmacological Studies: Embraced in pharmacology, DAF-2 diacetate emerges as a valuable asset for evaluating the efficacy of NO-related drugs and treatments. By gauging changes in fluorescence, scientists discern how various compounds impact nitric oxide production, aiding in the screening of potential drug candidates and unraveling their mechanisms of action. This application propels research forward, enhancing our comprehension of drug responses and facilitating the development of novel therapeutic avenues.
Disease Research: In the realm of disease investigation, DAF-2 diacetate emerges as a key player in exploring conditions where nitric oxide assumes a central role, such as cardiovascular diseases, neurodegenerative disorders, and inflammatory states. Tracking alterations in NO levels furnishes invaluable insights into disease mechanisms and the impacts of therapeutic interventions, fueling the creation of diagnostic tools and treatment strategies for these ailments.
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