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Cyanine5 hydrazide | 1427705-31-4
| Catelog Number | R05-0008 |
| Category | Hydrazides |
| Molecular Formula | C32H42Cl2N4O |
| Molecular Weight | 569.61 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| R05-0008 | -- | $-- |
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Product Introduction
Cyanine5 hydrazide is a reactive dye for the labeling of aldehydes and ketones, an analog of Cy5® hydrazide. This dye reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. Examples are proteins subjected to oxidative stress, glycosylated proteins pre-activated by periodate oxidation (including antobodies), and oligonucleotides with aldehyde moieties.
Chemical Information |
|
|---|---|
| Synonyms | [6-[3,3-dimethyl-2-[5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]hexanoylamino]azanium;dichloride |
| Purity | NMR 1H, HPLC-MS (95%) |
| IUPAC Name | [6-[3,3-dimethyl-2-[5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]hexanoylamino]azanium;dichloride |
| Canonical SMILES | CC1(C2=CC=CC=C2[N+](=C1C=CC=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)N[NH3+])(C)C)C)C.[Cl-].[Cl-] |
| InChI | InChI=1S/C32H40N4O.2ClH/c1-31(2)24-16-11-13-18-26(24)35(5)28(31)20-8-6-9-21-29-32(3,4)25-17-12-14-19-27(25)36(29)23-15-7-10-22-30(37)34-33;;/h6,8-9,11-14,16-21H,7,10,15,22-23,33H2,1-5H3;2*1H |
| InChI Key | RGKQQFSVYKQZDO-UHFFFAOYSA-N |
| Solubility | moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols) |
| Appearance | dark blue powder |
Product Specification |
|
|---|---|
| ε, L⋅mol-1⋅cm-1 | 250000 |
| Fluorescence Quantum Yield | 0.2 |
| Excitation | 646 |
| Emission | 662 |
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Computed Properties | |
|---|---|
| Hydrogen Bond Donor Count | 2 |
| Hydrogen Bond Acceptor Count | 4 |
| Rotatable Bond Count | 9 |
| Exact Mass | 568.2735674 g/mol |
| Monoisotopic Mass | 568.2735674 g/mol |
| Topological Polar Surface Area | 63Ų |
| Heavy Atom Count | 39 |
| Formal Charge | 0 |
| Complexity | 940 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 3 |
| Covalently-Bonded Unit Count | 3 |
| Compound Is Canonicalized | Yes |
Applications of Fluorescent Probes & Dyes
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