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BAPTA-TMFM | CAS 96315-11-6
| Catalog Number | A14-0134 |
| Category | Calcium, Chloride and Other indicators |
| Molecular Formula | C28H34N2O11 |
| Molecular Weight | 574.583 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
BAPTA-TMFM is a fluorescent probe that features a BAPTA-based calcium chelator integrated with a fluorophore, allowing it to participate in calcium ion detection and imaging applications. It exhibits specific excitation and emission properties that enable its use in fluorescence microscopy to monitor intracellular calcium dynamics. The compound's ability to conjugate with biomolecules through its reactive functional groups supports its incorporation into complex biosensing assays and cellular imaging workflows.
Chemical Information
Application
Computed Properties
Patents
Chemical Information
| Synonyms | methyl 2-[2-[2-[2-[bis(2-methoxy-2-oxoethyl)amino]-5-formylphenoxy]ethoxy]-N-(2-methoxy-2-oxoethyl)-4-methylanilino]acetate; N-[2-[2-[2-[BIS(2-METHOXY-2-OXOETHYL)AMINO]-5-FORMYLPHENOXY]ETHOXY]-4-METHYLPHENYL]-N-(2-METHOXY-2-OXOETHYL)-GLYCINE METHYL; Tetramethyl 5-formyl-5'-methylbis-(2-aminophenoxy-methylene)-N,N,N',N'-tetraacetate; 3-[2-[2-[Bis(2-oxo-2-methoxyethyl)amino]-5-methylphenoxy]ethoxy]-4-[bis(2-oxo-2-methoxyethyl)amino]benzaldehyde; methyl {[2-(2-{2-[bis(2-methoxy-2-oxoethyl)amino]-5-formylphenoxy}ethoxy)-4-methylphenyl](2-methoxy-2-oxoethyl)amino}acetate; METHYL 2-[2-(2-{2-[BIS(2-METHOXY-2-OXOETHYL)AMINO]-5-FORMYLPHENOXY}ETHOXY)(2-METHOXY-2-OXOETHYL)-4-METHYLANILINO]ACETATE |
| IUPAC Name | methyl 2-[2-[2-[2-[bis(2-methoxy-2-oxoethyl)amino]-5-formylphenoxy]ethoxy]-N-(2-methoxy-2-oxoethyl)-4-methylanilino]acetate |
| SMILES | CC1=CC(=C(C=C1)N(CC(=O)OC)CC(=O)OC)OCCOC2=C(C=CC(=C2)C=O)N(CC(=O)OC)CC(=O)OC |
| InChI | InChI=1S/C28H34N2O11/c1-19-6-8-21(29(14-25(32)36-2)15-26(33)37-3)23(12-19)40-10-11-41-24-13-20(18-31)7-9-22(24)30(16-27(34)38-4)17-28(35)39-5/h6-9,12-13,18H,10-11,14-17H2,1-5H3 |
| InChIKey | GKTVRWOCZLYLJV-UHFFFAOYSA-N |
| Appearance | Solid Powder |
Application
BAPTA-TMFM, a widely utilized calcium chelator in research, plays a crucial role in studying calcium dynamics within cells. Here are four key applications of BAPTA-TMFM:
Intracellular Calcium Measurement: Delving into live cells, BAPTA-TMFM serves as a vital tool for measuring intracellular calcium concentrations. Through its buffering action on calcium, this chelator enables researchers to meticulously monitor calcium fluctuations without disrupting normal cellular functions. This application is pivotal for unraveling the intricate involvement of calcium in processes such as muscle contraction, neurotransmitter release, and cell signaling.
Cell Viability Studies: In the realm of investigating cell death mechanisms, BAPTA-TMFM emerges as a key player in modulating intracellular calcium levels. By chelating calcium, this compound aids in shedding light on the role of calcium overload in apoptosis and necrosis. Such insights can be instrumental in identifying potential therapeutic targets for conditions marked by uncontrolled cell death, including neurodegenerative diseases.
Signal Transduction Research: At the forefront of exploring calcium-dependent signaling pathways, BAPTA-TMFM stands out as an indispensable tool for researchers. Leveraging this chelator, scientists can decipher the impact of calcium signals on diverse cellular responses, ranging from gene expression to enzyme activation. This intricate understanding of signaling pathways is paramount for the development of drugs capable of modulating cellular signaling in disease contexts.
Electrophysiological Studies: Within the realm of electrophysiological experimentation, BAPTA-TMFM plays a pivotal role in stabilizing the calcium environment of neuronal cells. Through precise control of calcium levels, researchers can delve into the electrophysiological properties of neurons, such as action potential firing and synaptic transmission. This in-depth analysis aids in unraveling the ionic mechanisms underlying neural activity and excitability, advancing our understanding of neuronal function.
Computed Properties
| XLogP3 | 3.3 |
| Hydrogen Bond Donor Count | 0 |
| Hydrogen Bond Acceptor Count | 13 |
| Rotatable Bond Count | 20 |
| Exact Mass | 574.21625990 g/mol |
| Monoisotopic Mass | 574.21625990 g/mol |
| Topological Polar Surface Area | 147Ų |
| Heavy Atom Count | 41 |
| Formal Charge | 0 |
| Complexity | 834 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
Patents
| Publication Number | Title | Priority Date |
|---|---|---|
| US-2010330584-A1 | Long wavelength fluorogenic intracellular ion indicators that are well retained in the cytosol | 2007-06-05 |
| US-2013096300-A1 | Long wavelength fluorogenic intracellular ion indicators that are well retained in the cytosol | 2007-06-05 |
| US-2014363839-A1 | Long wavelength fluorogenic intracellular ion indicators that are well retained in the cytosol | 2007-06-05 |
| US-8318502-B2 | Long wavelength fluorogenic intracellular ion indicators that are well retained in the cytosol | 2007-06-05 |
| US-9341631-B2 | Long wavelength fluorogenic intracellular ion indicators that are well retained in the cytosol | 2007-06-05 |
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