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Fluo-3 AM | CAS 121714-22-5
| Catalog Number | A14-0002 |
| Category | Calcium, Chloride and Other indicators |
| Molecular Formula | C51H50Cl2N2O23 |
| Molecular Weight | 1129.9 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Fluo-3 is a visable wavelength calcium indicator commonly used in flow cytometry (FC) and cell-based experiments to detect changes in intracellular calcium levels. Fluo-3 AM is cell-permeable and can be cleaved to release fluo-3 in cells.
Chemical Information
Product Specification
Application
| Synonyms | Fluo-3 Acetoxymethyl ester; N-[4-[6-[(acetyloxy)methoxy]-2,7-dichloro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine, (acetyloxy)methyl ester |
| Purity | ≥90% |
| Canonical SMILES | CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)Cl)OCOC(=O)C)Cl)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C |
| InChI | InChI=1S/C51H50Cl2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3 |
| InChIKey | PTPUOMXKXCCSEN-UHFFFAOYSA-N |
| Excitation | 488 nm |
| Emission | 526 nm |
| Storage | Store at -20°C |
Fluo-3 AM is a fluorescent calcium indicator commonly used in various biological and medical research applications. Here are some key applications of Fluo-3 AM:
Calcium Imaging in Neuroscience: Fluo-3 AM is widely used in neuroscience research to visualize and measure calcium dynamics in neurons. By loading cells with Fluo-3 AM, researchers can monitor calcium transients in real-time under a fluorescence microscope. This technique helps in understanding neural activity, synaptic transmission, and the overall function of neural networks.
Cardiomyocyte Studies: In cardiology research, Fluo-3 AM is employed to study calcium signaling in cardiomyocytes, the heart muscle cells. Calcium fluxes are critical for heart muscle contraction and the regulation of cardiac rhythms. Researchers use Fluo-3 AM to investigate the effects of drugs, diseases, or genetic modifications on calcium dynamics within heart cells, providing insights into cardiac physiology and pathophysiology.
Drug Screening: Fluo-3 AM is used in high-throughput screening assays to identify compounds that modulate calcium levels in cells. In pharmaceutical research, potential drugs are tested for their effects on calcium signaling pathways using Fluo-3 AM-loaded cells. This application is particularly valuable in discovering new therapeutics for disorders related to calcium dysregulation, such as certain cardiovascular and neurodegenerative diseases.
Cell Physiology Studies: Fluo-3 AM helps researchers study calcium-dependent processes in various cell types, including muscle cells, immune cells, and cancer cells. Calcium ions play central roles in numerous cellular functions, such as muscle contraction, cell migration, and apoptosis. By monitoring changes in intracellular calcium levels with Fluo-3 AM, scientists can unravel the mechanisms behind these vital cellular activities and their alterations in disease conditions.
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