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Rhodamine B hydrazide | 74317-53-6
| Catalog Number | A14-0036 |
| Category | Calcium, Chloride Indictors and Other indicators |
| Molecular Formula | C28H32N4O2 |
| Molecular Weight | 456.6 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A14-0036 | 100 mg | $239 |
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Product Introduction
Rhodamine B hydrazide is a water-soluble fluorescent probe used for the detection of copper, peroxynitrite, nitric oxide, hydrogen peroxide, glucose, diacetyl, and hemoglobin.
Chemical Information |
|
|---|---|
| Synonyms | 2-amino-3',6'-bis(diethylamino)-spiro[1H-isoindole-1,9'-[9H]xanthen]-3(2H)-one |
| Purity | ≥98% |
| IUPAC Name | 2-amino-3',6'-bis(diethylamino)spiro[isoindole-3,9'-xanthene]-1-one |
| Canonical SMILES | CCN(CC)C1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)N(CC)CC)C5=CC=CC=C5C(=O)N3N |
| InChI | InChI=1S/C28H32N4O2/c1-5-30(6-2)19-13-15-23-25(17-19)34-26-18-20(31(7-3)8-4)14-16-24(26)28(23)22-12-10-9-11-21(22)27(33)32(28)29/h9-18H,5-8,29H2,1-4H3 |
| InChI Key | WTDHTIVYKKLOTC-UHFFFAOYSA-N |
| Appearance | Solid Powder |
- Product Specification
- Application
| Excitation | 510 nm |
| Emission | 578 nm |
| Storage | Store at -20°C |
| Signal | Danger |
| GHS Hazard Statements | H302 (97.44%): Harmful if swallowed [Warning Acute toxicity, oral] H315 (100%): Causes skin irritation [Warning Skin corrosion/irritation] H318 (97.44%): Causes serious eye damage [Danger Serious eye damage/eye irritation] H335 (100%): May cause respiratory irritation [Warning Specific target organ toxicity, single exposure; Respiratory tract irritation] |
| Precautionary Statement Codes | P261, P264, P264+P265, P270, P271, P280, P301+P317, P302+P352, P304+P340, P305+P354+P338, P317, P319, P321, P330, P332+P317, P362+P364, P403+P233, P405, and P501 (The corresponding statement to each P-code can be found at the GHS Classification page.) |
Rhodamine B hydrazide is a fluorescent dye used in various biological and industrial applications due to its bright fluorescence and high sensitivity. Here are some key applications of Rhodamine B hydrazide:
Fluorescent Labeling: Rhodamine B hydrazide is commonly used as a fluorescent label in biological assays and imaging studies. It can be conjugated to biomolecules such as proteins, nucleic acids, and antibodies to visualize cellular structures and processes. This enables researchers to study cellular localization, protein interactions, and other dynamic biological events with high precision.
Live Cell Imaging: Rhodamine B hydrazide is valuable in live cell imaging applications where it can selectively stain and track live cells over time. Its bright fluorescence and compatibility with various microscopy techniques make it ideal for observing cell behavior, migration, and division in real-time. This information is crucial for understanding developmental biology, disease progression, and therapeutic responses.
Environmental Monitoring: Rhodamine B hydrazide is used in environmental monitoring to detect and quantify pollutants in water and soil samples. Its fluorescence properties allow for sensitive detection of trace amounts of contaminants such as heavy metals and pesticides. This application is essential for environmental protection and ensuring compliance with safety regulations.
Flow Cytometry: In flow cytometry, Rhodamine B hydrazide is utilized as a fluorescent dye for labeling cells and analyzing their properties. By staining cells with Rhodamine B hydrazide, researchers can measure various cellular parameters such as viability, apoptosis, and cell cycle status. This technique is widely used in immunology, cancer research, and clinical diagnostics to gain insights into cellular function and health.
Computed Properties | |
|---|---|
| XLogP3 | 4.7 |
| Hydrogen Bond Donor Count | 1 |
| Hydrogen Bond Acceptor Count | 5 |
| Rotatable Bond Count | 6 |
| Exact Mass | 456.25252628 g/mol |
| Monoisotopic Mass | 456.25252628 g/mol |
| Topological Polar Surface Area | 62Ų |
| Heavy Atom Count | 34 |
| Formal Charge | 0 |
| Complexity | 687 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
Patents
| Publication Number | Title | Priority Date |
|---|---|---|
| CN-114778644-A | A kind of preparation method of iridium (III) complex sensitized NiO photocathode for detecting Hg2+ | 2022-04-11 |
| CN-114539274-A | Single factor induced photochromic sensing material and preparation method and application thereof | 2022-03-15 |
| CN-114563384-A | A kind of fluorescence detection method of copper ion | 2022-02-28 |
| CN-113603701-B | A colorimetric/fluorescent probe for detecting hypochlorite ion and its preparation method and application | 2021-08-11 |
| CN-113637027-B | Phenyl triazole dicarboxylic acid-rhodamine B derivative fluorescent probe and preparation method and application thereof | 2021-07-22 |
Applications of Fluorescent Probes & Dyes
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