Live Imaging of the Extracellular Matrix With a Glycan-Binding Fluorophore

June 9th, 2026 10:00 AM EDT

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4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid disodium salt

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4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid disodium salt

4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid disodium salt | CAS 51023-76-8

Catalog Number	A14-0138
Category	Calcium, Chloride and Other indicators
Molecular Formula	C17H12N2Na2O7S3
Molecular Weight	498.45

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Product Introduction

4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid disodium salt is a fluorescent dye featuring a stilbene chromophore, known for its conjugated aromatic system that facilitates strong absorption and emission properties. This compound contains an isothiocyanate reactive group, enabling covalent conjugation to amine groups in proteins and other biomolecules, making it suitable for various labeling applications. It is often used in fluorescence microscopy and flow cytometry, where its spectral properties allow for effective visualization and tracking of biological molecules within complex systems.

Chemical Information
Application

Chemical Information

Synonyms	SITS
SMILES	CC(=O)NC1=CC(=C(C=C1)C=CC2=C(C=C(C=C2)N=C=S)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+]
InChI	InChI=1S/C17H14N2O7S3.2Na/c1-11(20)19-15-7-5-13(17(9-15)29(24,25)26)3-2-12-4-6-14(18-10-27)8-16(12)28(21,22)23;;/h2-9H,1H3,(H,19,20)(H,21,22,23)(H,24,25,26);;/q;2*+1/p-2/b3-2+;;
InChIKey	NPAWAMRXPHRVQY-WTVBWJGASA-L
Appearance	Solid Powder

Application

4-Acetamido-4’-isothiocyanatostilbene-2,2’-disulfonic acid disodium salt is a fluorescent dye primarily utilized in various scientific applications. Here are some key applications:

Fluorescence Microscopy: 4-Acetamido-4’-isothiocyanatostilbene-2,2’-disulfonic acid disodium salt is highly effective in fluorescence microscopy for labeling and visualizing cellular structures. It binds to specific cellular components, enabling researchers to track and observe cellular processes in real-time. This application is particularly valuable in cell biology and histology for studying the localization and dynamics of biomolecules.

Membrane Protein Labeling: The compound is extensively used to label membrane proteins, facilitating the study of their distribution and function within cell membranes. By attaching to specific amino acid residues on proteins, it allows for detailed visualization and analysis using fluorescence spectroscopy techniques. This is crucial for understanding membrane protein behavior, interactions, and their roles in cellular signaling.

Flow Cytometry: In flow cytometry assays, 4-Acetamido-4’-isothiocyanatostilbene-2,2’-disulfonic acid disodium salt can be utilized as a fluorescent marker to differentiate and quantify various cell populations. Its fluorescent properties enable precise detection and analysis of specific cell types based on surface markers. This application is essential in immunology, hematology, and cancer research for cell sorting, phenotyping, and diagnostics.

Anion Transport Studies: The dye is used in biophysical and biochemical experiments to study anion transport across biological membranes. By monitoring fluorescence changes, researchers can assess the dynamics of ion transport processes and their regulation. This information is pivotal in examining cellular ion homeostasis and the function of transport proteins, providing insights into physiological and pathological conditions.