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Fluo-3 potassium salt | 853400-67-6
| Catalog Number | A14-0030 |
| Category | Calcium, Chloride Indictors and Other indicators |
| Molecular Formula | C36H25Cl2K5N2O13 |
| Molecular Weight | 960.0 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A14-0030 | -- | $-- |
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Product Introduction
Fluo-3 is a fluorescent indicator for intracellular Ca2+ that is used in flow cytometry and cell-based experiments. Fluo-3 produces fluorescence with an emission maximum at 525 nm upon binding to calcium.
Chemical Information |
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|---|---|
| Related CAS | 123632-39-3 (free acid) |
| Synonyms | N-[2-[2-[2-[bis(carboxymethyl)amino]-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy]ethoxy]-4-methylphenyl]-N-(carboxymethyl)-glycine, pentapotassium salt |
| Purity | ≥90% |
| Canonical SMILES | CC1=CC(=C(C=C1)N(CC(=O)[O-])CC(=O)[O-])OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)Cl)[O-])Cl)N(CC(=O)[O-])CC(=O)[O-].[K+].[K+].[K+].[K+].[K+] |
| InChI | InChI=1S/C36H30Cl2N2O13.5K/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36;;;;;/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50);;;;;/q;5*+1/p-5 |
| InChI Key | HESJPJDGZLKNAU-UHFFFAOYSA-I |
| Appearance | Solid Powder |
- Product Specification
- Application
| Excitation | 488 nm |
| Emission | 526 nm |
| Storage | Store at -20°C |
Fluo-3 potassium salt, a fluorescent dye renowned for monitoring calcium levels in biological cells, finds diverse applications in various fields. Here are four key applications of Fluo-3 potassium salt:
Cell-based Calcium Imaging: Widely utilized in fluorescence microscopy, Fluo-3 potassium salt plays a pivotal role in visualizing calcium ion concentrations within live cells. When bound to calcium, Fluo-3 exhibits a remarkable increase in fluorescence, enabling researchers to meticulously track calcium signaling events in real-time. This application is indispensable for unraveling the intricacies of various cellular processes, including signal transduction, muscle contraction, and neurotransmitter release.
Pharmacological Screening: In the realm of drug discovery, Fluo-3 potassium salt serves as a potent tool in high-throughput screening assays designed to pinpoint compounds modulating calcium signaling pathways. By scrutinizing fluctuations in fluorescence intensity, researchers can gauge the efficacy and potency of potential drug candidates. This technique stands at the forefront of developing novel therapeutics targeting calcium-mediated processes, particularly impacting cardiac and neuronal functions.
Neuroscience Research: Delving deep into the realm of neuroscience, Fluo-3 potassium salt emerges as a valuable asset for probing calcium dynamics in neurons and glial cells. By leveraging Fluo-3, scientists embark on a journey to explore synaptic activity, neuroplasticity, and neuronal development, unraveling the complex web of brain function and shedding light on the underlying mechanisms of neurological disorders.
Cardiovascular Studies: In the realm of cardiovascular research, Fluo-3 potassium salt takes center stage in scrutinizing calcium flux within cardiac myocytes and vascular smooth muscle cells. Monitoring calcium levels using Fluo-3 aids in deciphering the regulation of heartbeats, vascular tone, and responses to cardiovascular drugs. This application holds significant weight in the quest to develop cutting-edge treatments for heart diseases and hypertension, pushing the boundaries of cardiovascular medicine.
Applications of Fluorescent Probes & Dyes
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